Wong Andrea Li-Ann, Xiang Xiaoqiang, Ong Pei Shi, Mitchell Ee Qin Ying, Syn Nicholas, Wee Ian, Kumar Alan Prem, Yong Wei Peng, Sethi Gautam, Goh Boon Cher, Ho Paul Chi-Lui, Wang Lingzhi
Cancer Science Institute of Singapore, National University of Singapore, Singapore 117599, Singapore.
Department of Haematology-Oncology, National University Health System, Singapore 119228, Singapore.
Pharmaceutics. 2018 Nov 8;10(4):221. doi: 10.3390/pharmaceutics10040221.
In the last decade, the tremendous improvement in the sensitivity and also affordability of liquid chromatography-tandem mass spectrometry (LC-MS/MS) has revolutionized its application in pharmaceutical analysis, resulting in widespread employment of LC-MS/MS in determining pharmaceutical compounds, including anticancer drugs in pharmaceutical research and also industries. Currently, LC-MS/MS has been widely used to quantify small molecule oncology drugs in various biological matrices to support preclinical and clinical pharmacokinetic studies in R&D of oncology drugs. This mini-review article will describe the state-of-the-art LC-MS/MS and its application in rapid quantification of small molecule anticancer drugs. In addition, efforts have also been made in this review to address several key aspects in the development of rapid LC-MS/MS methods, including sample preparation, chromatographic separation, and matrix effect evaluation.
在过去十年中，液相色谱-串联质谱（LC-MS/MS）的灵敏度和可承受性都有了巨大提高，彻底改变了其在药物分析中的应用，使得LC-MS/MS在测定药物化合物（包括药物研发和制药行业中的抗癌药物）方面得到广泛应用。目前，LC-MS/MS已被广泛用于定量分析各种生物基质中的小分子肿瘤药物，以支持肿瘤药物研发中的临床前和临床药代动力学研究。这篇小型综述文章将介绍最先进的LC-MS/MS及其在快速定量小分子抗癌药物中的应用。此外，本综述还努力探讨了快速LC-MS/MS方法开发中的几个关键方面，包括样品制备、色谱分离和基质效应评估。
