Martins Polyana Kelly, da Cunha Bárbara Andrade Dias Brito, Kobayshi Adilson Kenji, Molinari Hugo Bruno Correa
Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE), Brazilian Agricultural Research Corporation (EMBRAPA), Parque Estação Biológica, Brasília, DF, Brazil.
Methods Mol Biol. 2019;1864:49-65. doi: 10.1007/978-1-4939-8778-8_4.
Setaria viridis is an emerging model for C4 species, and it is an important model to validate some genes for further C4 crop transformation, such as sugarcane, maize, and wheat. Here, we describe two protocols for stable transformation of S. viridis mediated by Agrobacterium tumefaciens with three different reporter genes and two selectable markers. Routine transformation efficiency reaching 29% was achieved using embryogenic callus in S. viridis (accession A10.1). Alternatively, we developed a transformation method by floral dip with 0.6% efficiency. The developed protocols could be useful for genetic and genomics studies of important food-feed-fiber-fuel C4 crops.
绿色狗尾草是一种新兴的C4植物模式，也是验证一些基因以用于进一步C4作物转化（如甘蔗、玉米和小麦）的重要模式。在此，我们描述了两种通过根癌农杆菌介导的绿色狗尾草稳定转化方案，使用了三种不同的报告基因和两种选择标记。使用绿色狗尾草（种质A10.1）的胚性愈伤组织实现了常规转化效率达到29%。另外，我们开发了一种通过花浸法的转化方法，效率为0.6%。所开发的方案可能对重要的粮食-饲料-纤维-燃料C4作物的遗传和基因组学研究有用。
