Wu Chuanyin, Sui Yi
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Methods Mol Biol. 2019;1864:95-103. doi: 10.1007/978-1-4939-8778-8_7.
Genetic transformation plays a key role in deciphering regulation of agronomic traits at molecular level in rice, a model monocot cereal crop. Here we describe an efficient and fast protocol for producing transgenic japonica rice plants using the Agrobacterium-mediated transformation method. The protocol simplifies medium compositions and transformation steps and can be easily followed by a lab technician with little tissue culture experience. Using this protocol, we have transformed thousands of gene constructs in the past 10 years and edited hundreds of genes with the CRISPR-Cas9 system recently.
遗传转化在解析单子叶模式谷类作物水稻农艺性状的分子调控方面发挥着关键作用。在此，我们描述了一种利用农杆菌介导转化法高效快速培育转基因粳稻植株的方案。该方案简化了培养基成分和转化步骤，几乎没有组织培养经验的实验室技术人员也能轻松遵循。使用该方案，在过去10年里我们已转化了数千个基因构建体，最近还用CRISPR-Cas9系统编辑了数百个基因。
