Forslind B
Department of Medical Biophysics, Karolinska Institutet, Stockholm, Sweden.
Scanning Microsc. 1988 Jun;2(2):959-76.
Dermatological papers comprising scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analysis data published 1983 through 1986 in international journals are reviewed, as an update to our 1984 paper on Clinical applications of scanning electron microscopy and X-ray microanalysis in dermatology. The present paper not only deals with a review of recent publications in this area but also presents the application of microincineration to hair and cryosectioned freeze-dried skin specimens. Examples of the increased contrast obtained in hair cross sections are presented and a discussion on the feasibility of microincineration at analysis of hair and skin cross sections is given. Particle probe analysis (EDX: energy dispersive X-ray analysis and PMP: proton microprobe analysis) as applied to hair and skin samples are presented with stress put on the proton probe analysis. The complementarity of EDX and PMP is demonstrated and future applications are suggested.
本文回顾了1983年至1986年发表在国际期刊上的包含扫描电子显微镜(SEM)和能量色散X射线(EDX)分析数据的皮肤病学论文,作为我们1984年关于扫描电子显微镜和X射线微分析在皮肤病学中的临床应用论文的更新。本文不仅对该领域的近期出版物进行了综述,还介绍了微灰化在毛发和冷冻切片冻干皮肤标本中的应用。给出了在毛发横切面上获得更高对比度的示例,并对微灰化在毛发和皮肤横切面分析中的可行性进行了讨论。介绍了应用于毛发和皮肤样本的粒子探针分析(EDX:能量色散X射线分析和PMP:质子微探针分析),重点介绍了质子探针分析。证明了EDX和PMP的互补性,并提出了未来的应用建议。