Department of Chemical and Food Engineering, Federal University of Santa Catarina, Florianópolis, SC 88040-900, Brazil.
Central Laboratory of Structural Molecular Biology (CEBIME), Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil.
Int J Biol Macromol. 2019 Feb 1;122:653-658. doi: 10.1016/j.ijbiomac.2018.10.171. Epub 2018 Oct 25.
Alternatives to improve the stability and activity of enzymes have been rising in the last years due to the potential industrial application of these catalysts. However, the enzymes characteristics in terms of stability and catalytic efficiency can reduce, in some cases, due to the reaction conditions. Due to a lack in the literature concerning structural information related to the new commercial Eversa® Transform 2.0 enzyme (NS-40116) we investigated the conformational structure by spectroscopic and mass spectrometry techniques after exposure in permanent magnetic flux density (0.7 and 1.34 T) in recirculation mode (1, 2, and 4 h) at 0.06 L·min. The influence of pH on the enzymatic solution associated with the magnetic flux (pH 5, 7, and 9) was also evaluated. Under the best reaction condition (pH 7 after 4 h in a recirculation mode at 1.34 T), enzyme activity 77% higher than the control sample was obtained. Mass spectrometry techniques showed changes in the NS-40116 tertiary structure. Thus, the application of magnetic fields as an enzymatic pre-treatment showed to be a promising technique and a viable alternative to increase the enzymatic activity since it is a low cost, environmentally friendly, and ease operation process.
近年来，由于这些催化剂具有潜在的工业应用价值，提高酶稳定性和活性的方法不断涌现。然而，由于反应条件的影响，某些情况下，酶的稳定性和催化效率特性会降低。由于缺乏有关新型商业 Eversa® Transform 2.0 酶（NS-40116）结构信息的文献，我们通过光谱和质谱技术研究了在循环模式下（1、2 和 4 小时）暴露于恒定磁场密度（0.7 和 1.34 T）下的构象结构，流速为 0.06 L·min。还评估了 pH 值对与磁场相关的酶溶液的影响（pH 值为 5、7 和 9）。在最佳反应条件下（pH 值为 7，在 1.34 T 下循环模式 4 小时），获得了比对照样品高 77%的酶活性。质谱技术显示 NS-40116 三级结构发生了变化。因此，磁场作为酶预处理的应用被证明是一种有前途的技术，也是提高酶活性的可行替代方法，因为它具有低成本、环保和易于操作的特点。
