Department of Chemical Engineering, School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Selangor, Malaysia; Monash-Industry Plant Oils Research Laboratory (MIPO), Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Selangor, Malaysia.
Department of Chemical Engineering, School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Selangor, Malaysia; Monash-Industry Plant Oils Research Laboratory (MIPO), Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Selangor, Malaysia.
Int J Biol Macromol. 2024 Sep;276(Pt 1):133817. doi: 10.1016/j.ijbiomac.2024.133817. Epub 2024 Jul 11.
Ultrasound technology has emerged as a promising tool for enhancing enzymatic biodiesel production, yet the cavitation effect induced can compromise enzyme stability. This study explored the efficiency of polyols in enhancing lipase stability under ultrasound conditions to further improve biodiesel yield. The incorporation of sorbitol resulted in the highest fatty acid methyl ester (FAME) content in the ultrasound-assisted biodiesel production catalyzed by Eversa® Transform 2.0 among the investigated polyols. Furthermore, sorbitol enhanced the stability of the lipase, allowing it to tolerate up to 100 % ultrasound amplitude, compared to 60 % amplitude in its absence. Enzyme activity assays revealed that sorbitol preserved 99 % of the lipase activity, in contrast to 84 % retention observed without sorbitol under an 80 % ultrasound amplitude. Circular dichroism (CD) and fluorescence spectroscopy analyses confirmed that sorbitol enhanced lipase rigidity and preserved its conformational structure under ultrasound exposure. Furthermore, employing a stepwise methanol addition strategy in ultrasound-assisted reactions with sorbitol achieved an 81.2 wt% FAME content in 8 h with only 0.2 wt% enzyme concentration. This promising result highlights the potential of sorbitol as a stabilizing agent in ultrasound-assisted enzymatic biodiesel production, offering a viable approach for enhancing biodiesel yield and enzyme stability in industrial applications.
超声技术已成为提高酶法生物柴油生产的有前途的工具，但所诱导的空化效应可能会影响酶的稳定性。本研究探讨了多元醇在超声条件下提高脂肪酶稳定性以进一步提高生物柴油产率的效率。在研究的多元醇中，山梨醇的加入使 Eversa® Transform 2.0 催化的超声辅助生物柴油生产中的脂肪酸甲酯 (FAME) 含量最高。此外，山梨醇增强了脂肪酶的稳定性，使其能够在高达 100%的超声幅度下耐受，而在没有山梨醇的情况下，其耐受幅度为 60%。酶活性测定表明，与在 80%超声幅度下没有山梨醇时观察到的 84%保留率相比，山梨醇保留了 99%的脂肪酶活性。圆二色性 (CD) 和荧光光谱分析证实，山梨醇增强了脂肪酶的刚性，并在超声暴露下保持了其构象结构。此外，在使用山梨醇的超声辅助反应中采用逐步添加甲醇的策略，仅用 0.2wt%的酶浓度在 8 小时内即可达到 81.2wt%的 FAME 含量。这一有希望的结果突出了山梨醇作为超声辅助酶法生物柴油生产中稳定剂的潜力，为提高生物柴油产率和工业应用中酶的稳定性提供了一种可行的方法。
