Smart Y C, Millard P R
Department of Histopathology, John Radcliffe Hospital, Headington, Oxford, England.
Ultrastruct Pathol. 1988 May-Jun;12(3):291-9. doi: 10.3109/01913128809098041.
Two indirect immunocytochemical techniques using different markers, namely peroxidase and gold, were applied to ultrathin sections of liver biopsies to detect alpha-1-antitrypsin. The blocks used were taken from the routine electron microscopy files and had been processed optimally for maximum ultrastructural preservation. The immunogold technique provided the best method for localizing alpha-1-antitrypsin and was associated with ultrastructural preservation equivalent to that seen in routinely processed liver biopsies. The procedure may be a useful adjunct to understanding the pathogenesis of alpha-1-antitrypsin deficiency and in recognizing further variants of this disorder.
两种使用不同标记物（即过氧化物酶和金）的间接免疫细胞化学技术被应用于肝活检的超薄切片，以检测α-1-抗胰蛋白酶。所用的组织块取自常规电子显微镜档案，并且已经进行了最佳处理以实现最大程度的超微结构保存。免疫金技术为定位α-1-抗胰蛋白酶提供了最佳方法，并且与常规处理的肝活检中所见的超微结构保存相当。该方法可能有助于理解α-1-抗胰蛋白酶缺乏症的发病机制以及识别该疾病的更多变体。
