Romanov Yu A, Volgina N E, Dugina T N, Kabaeva N V, Sukhikh G T
National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation, Moscow, Russia.
CryoCenter Cord Blood Bank, Moscow, Russia.
Bull Exp Biol Med. 2018 Nov;166(1):124-129. doi: 10.1007/s10517-018-4300-x. Epub 2018 Nov 12.
Production of microvesicles in culture of human umbilical cord multipotent mesenchymal stromal cells was studied and comparative analysis of the expression of some surface molecules (clusters of differentiation, CD) was performed. It was found that the mesenchymal stromal cells produce microvesicles in the amount sufficient for their detection by flow cytometry. Parallel analysis of the phenotypes of maternal mesenchymal stromal cells and secreted microvesicles revealed identical expression of surface molecules CD13, CD29, CD44, CD54, CD71, CD73, CD90, CD105, CD106, and HLA-I. The concentration of microvesicles in the conditioned medium was 17.9±4.6×10/ml; i.e. one cell produced ~40-50 (44.7±11.5) microvesicles over 2 days in culture.
研究了人脐带多能间充质基质细胞培养中微泡的产生，并对一些表面分子（分化簇，CD）的表达进行了比较分析。发现间充质基质细胞产生的微泡数量足以通过流式细胞术检测到。对母体间充质基质细胞和分泌微泡的表型进行平行分析，发现表面分子CD13、CD29、CD44、CD54、CD71、CD73、CD90、CD105、CD106和HLA-I的表达相同。条件培养基中微泡的浓度为17.9±4.6×10/ml；即一个细胞在培养2天内产生约40-50（44.7±11.5）个微泡。
