Alperina Elizaveta, Idova Galina, Zhukova Elena, Zhanaeva Svetlana, Kozhemyakina Rimma
Federal State Budgetary Scientific Institution "Scientific Research Institute of Physiology and Basic Medicine", Timakova Street, 4, Novosibirsk, Russia.
Federal State Budgetary Scientific Institution "Scientific Research Institute of Physiology and Basic Medicine", Timakova Street, 4, Novosibirsk, Russia; Novosibirsk State University, Pirogova Street, 2, 630090, Novosibirsk, Russia.
Neurosci Lett. 2019 Jan 23;692:193-198. doi: 10.1016/j.neulet.2018.11.012. Epub 2018 Nov 10.
The present study examined the content of cytokines (IL-1β, IL-2, IL-6, IL-10) in the brain structures (the hypothalamus, striatum, frontal cortex, and hippocampus) in two rat lines selected for differences in fear-induced aggression at 2, 4, and 24 h after a peripheral injection of saline or lipopolysaccharide (LPS, 250 μg/kg). LPS stimulation elevated cytokine activity above baseline levels in both aggressive and nonaggressive rats, but the pattern, time course of cytokine changes, and their regional characteristics varied according to the animal aggressiveness. After LPS administration, aggressive rats showed increased levels of IL-1β in the hypothalamus at 2 and 4 h and in the frontal cortex at 4 and 24 h compared to LPS-treated nonaggressive line. IL-2 was increased in the frontal cortex and striatum of aggressive rats within 24 h, while IL-6 elevation in the hypothalamus was found at 4 h and in the frontal cortex at 2 and 4 h. In the hippocampus, the levels of IL-1β, IL-2, and IL-6 were lower in LPS-treated aggressive rats than in nonaggressive animals. The levels of anti-inflammatory cytokine IL-10 were also decreased in all brain structures of aggressive rats receiving LPS. The results indicate that genetic predisposition to increased aggression is associated with a time and region-dependent changes in the levels of pro- and anti-inflammatory cytokines.
本研究检测了两组因恐惧诱导攻击行为存在差异而挑选出的大鼠品系，在经外周注射生理盐水或脂多糖（LPS，250μg/kg）后2小时、4小时和24小时，其脑结构（下丘脑、纹状体、额叶皮质和海马体）中细胞因子（IL-1β、IL-2、IL-6、IL-10）的含量。LPS刺激使攻击性和非攻击性大鼠的细胞因子活性均高于基线水平，但细胞因子变化的模式、时间进程及其区域特征因动物的攻击性而异。给予LPS后，与接受LPS处理的非攻击性品系相比，攻击性大鼠下丘脑在2小时和4小时、额叶皮质在4小时和24小时时IL-1β水平升高。攻击性大鼠额叶皮质和纹状体中的IL-2在24小时内升高，而下丘脑的IL-6在4小时时升高，额叶皮质在2小时和4小时时升高。在海马体中，接受LPS处理的攻击性大鼠的IL-1β、IL-2和IL-6水平低于非攻击性动物。接受LPS的攻击性大鼠所有脑结构中的抗炎细胞因子IL-10水平也降低。结果表明，攻击性增加的遗传易感性与促炎和抗炎细胞因子水平的时间和区域依赖性变化有关。
