Gao Yuan, Yao Yao, Liang Xin, Tang Jing, Ma Jing, Qi Ying-Qiang, Huang Chun-Xia, Zhang Yang, Chen Lin-Mu, Chao Feng-Lei, Zhang Lei, Luo Yan-Min, Xiao Qian, Du Lian, Xiao Qian, Wang San-Rong, Tang Yong
Department of Geriatrics, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, PR China; Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, PR China; Laboratory of Stem Cells and Tissue Engineering, Chongqing Medical University, Chongqing 400016, PR China.
Department of Geriatrics, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, PR China; Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, PR China; Laboratory of Stem Cells and Tissue Engineering, Chongqing Medical University, Chongqing 400016, PR China.
Neurosci Lett. 2019 Feb 16;694:104-110. doi: 10.1016/j.neulet.2018.11.013. Epub 2018 Nov 10.
To explore the pathogenesis of depression and the possible mechanism of the effects of selective serotonin reuptake inhibitors (SSRIs) on the myelinated fibers and myelin sheaths in the white matter during the antidepressant action of fluoxetine.
In this study, Sprague Dawley (SD) rats were divided into a Control group, a group treated with CUS and no drugs (CUS/Standard group) and a group treated with CUS and fluoxetine (CUS/FLX group). The CUS/FLX group was treated with fluoxetine at dose of 5 mg/kg for 21 days. The white matter volume, the myelinated fiber parameters and the myelin sheath volume in the white matter were calculated from transmission electron microscope images through unbiased stereological methods.
The total volume and total length of myelinated fibers;and mean volume of white matter of the CUS/Standard group were significantly decreased compared to values from the control group (p = 0.025, p = 0.007, p = 0.000), whereas no significant differences in these stereological parameters were found between the CUS/Standard and CUS/FLX groups (p > 0.05).
Fluoxetine successfully treated depression-like behavior but had no effects on the white matter or its component myelinated fibers in the CUS rat model of depression.
探讨抑郁症的发病机制以及在氟西汀抗抑郁作用过程中选择性5-羟色胺再摄取抑制剂（SSRIs）对白质中髓鞘纤维和髓鞘产生影响的可能机制。
在本研究中，将Sprague Dawley（SD）大鼠分为对照组、慢性不可预知应激（CUS）且未用药组（CUS/标准组）和CUS且使用氟西汀组（CUS/氟西汀组）。CUS/氟西汀组以5mg/kg的剂量给予氟西汀，持续21天。通过无偏倚体视学方法，根据透射电子显微镜图像计算白质体积、髓鞘纤维参数和白质中的髓鞘体积。
与对照组相比，CUS/标准组的髓鞘纤维总体积和总长度以及白质平均体积显著降低（p = 0.025，p = 0.007，p = 0.000），而CUS/标准组和CUS/氟西汀组之间在这些体视学参数上未发现显著差异（p > 0.）。
在CUS大鼠抑郁模型中，氟西汀成功治疗了抑郁样行为，但对白质及其组成成分髓鞘纤维没有影响。
