Department of Urology, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa, Japan.
Department of Urology, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa, Japan.
Int J Infect Dis. 2019 Jan;78:148-154. doi: 10.1016/j.ijid.2018.11.003. Epub 2018 Nov 10.
To examine the association between human papillomavirus (HPV) infection and penile cancer among Japanese patients.
Thirty-four patients with penile cancer were enrolled in this study. DNA was extracted from paraffin-embedded tumor tissue samples, and HPV-DNA tests and genotyping were performed. For all of the samples, in situ hybridization (ISH) was performed to locate HPV-DNA in tumor tissue. Furthermore, expression levels of p16-INK4a, mini-chromosome maintenance protein 7(mcm-7), HPV-L1, and Ki-67 were analyzed using immunohistochemical methods.
HPV and high-risk (HR)-HPV were detected in 14 (41.1%; 95% confidence interval (CI) 24.6-57.7%) and 12 (35.2%; 95% CI 19.2-51.4%) cases, respectively. HPV16 was the most frequently detected HPV type. Among the HR-HPV-positive cases, a punctate HR-HPV-DNA signal pattern was detected by ISH in tumor cell nuclei. P16-INK4a was expressed in 66.7% (95% CI 42.8-90.1%) of HR-HPV-positive cases and was significantly more frequent and stronger in HR-HPV-positive cases than in HPV-negative cases. There was no significant difference in the occurrence or distribution of mcm-7 or Ki-67 expression between HPV-positive and HPV-negative cases. HPV-L1 expression was not observed in any of the cases examined.
HPV infection may have had an etiological role in 41% of the examined cases of penile cancer in Japan.
研究人乳头瘤病毒（HPV）感染与日本患者阴茎癌之间的关系。
本研究纳入了 34 例阴茎癌患者。从石蜡包埋的肿瘤组织样本中提取 DNA，进行 HPV-DNA 检测和基因分型。对所有样本进行原位杂交（ISH），以确定肿瘤组织中的 HPV-DNA 位置。此外，采用免疫组织化学方法分析 p16-INK4a、微小染色体维持蛋白 7（mcm-7）、HPV-L1 和 Ki-67 的表达水平。
在 14 例（41.1%；95%置信区间[CI]：24.6%至 57.7%）和 12 例（35.2%；95%CI：19.2%至 51.4%）患者中检测到 HPV 和高危型（HR）-HPV。HPV16 是最常见的 HPV 类型。在 HR-HPV 阳性病例中，ISH 检测到肿瘤细胞核中存在点状 HR-HPV-DNA 信号模式。p16-INK4a 在 66.7%（95%CI：42.8%至 90.1%）的 HR-HPV 阳性病例中表达，且在 HR-HPV 阳性病例中表达更为频繁且更强，与 HPV 阴性病例相比差异有统计学意义。HPV 阳性和 HPV 阴性病例的 mcm-7 或 Ki-67 表达的发生或分布无显著差异。在检查的病例中未观察到 HPV-L1 表达。
HPV 感染可能在日本 41%的阴茎癌病例中起病因作用。
