Department of Chemistry, Gandhigram Rural Institute (Deemed to be University), Gandhigram, 624 302, India.
Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Mater Sci Eng C Mater Biol Appl. 2019 Jan 1;94:778-787. doi: 10.1016/j.msec.2018.10.021. Epub 2018 Oct 6.
Three metal(II) complexes [CoLCl], [CuLCl] and [ZnLCl] {L = 2‑chloro‑3‑((3‑dimethylamino)propylamino)naphthalene‑1,4‑dione} have been synthesized and characterized using analytical, thermal and spectral techniques (FT-IR, UV-Vis, ESR and ESI-MS). The structure of the L has been confirmed by single crystal XRD study. The complexes show good binding propensity to bovine serum albumin (BSA) having relatively higher binding constant values (10 M) than the ligand. Fluorescence spectral studies indicate that [CoLCl] binds relatively stronger with CT DNA through intercalative mode, exhibiting higher binding constant (2.22 × 10 M). Agarose gel electrophoresis run on plasmid DNA (pUC18) prove that all the complexes showed efficient DNA cleavage via hydroxyl radical mechanism. The complexes were identified as potent anticancer agents against two human cancer cell lines (MCF7 and A549) by comparing with cisplatin. Co(II) complex demonstrated greater cytotoxicity against MCF7 and A549 cells with IC values at 19 and 22 μM, respectively.
三种金属（II）配合物[CoLCl]，[CuLCl]和[ZnLCl]（L=2-氯-3-（（3-二甲基氨基）丙基）氨基）萘-1,4-二酮）已通过分析，热和光谱技术（FT-IR，UV-Vis，ESR 和 ESI-MS）进行了合成和表征。通过单晶 XRD 研究证实了 L 的结构。该配合物与牛血清白蛋白（BSA）具有良好的结合倾向，其结合常数（10 M）高于配体。荧光光谱研究表明，[CoLCl]通过插入模式与 CT DNA 结合相对较强，表现出较高的结合常数（2.22×10 M）。琼脂糖凝胶电泳在质粒 DNA（pUC18）上运行证明，所有配合物均通过羟基自由基机制表现出有效的 DNA 切割活性。与顺铂相比，这些配合物被鉴定为两种人类癌细胞系（MCF7 和 A549）的有效抗癌剂。Co（II）配合物对 MCF7 和 A549 细胞的细胞毒性更强，IC 值分别为 19 和 22 μM。
