通过局部使用免疫抑制剂提高兔角膜移植存活率。

Increase of corneal graft survival by use of topically immunosuppressive agents in rabbits.

作者信息

Golubović S, Radmanović B Z

机构信息

University Eye Clinic, Medical Faculty, Belgrade, Yugoslavia.

出版信息

Graefes Arch Clin Exp Ophthalmol. 1988;226(3):288-90. doi: 10.1007/BF02181198.

DOI:10.1007/BF02181198

PMID:3042525

Abstract

Corneal graft survival in rabbits was significantly prolonged by topical treatment of the recipient eye with cyclophosphamide (0.1% in sterile isotonic NaCl) and methotrexate (0.1% in arachis oil) applied four times daily for 4 weeks. A control group of animals was treated with dexamethasone in the same way. The mean graft survival time was 52 +/- 18.5 days in the group treated with dexamethasone and 80 +/- 18.2 and 73 +/- 23.5, respectively, in the groups treated with cyclophosphamide and methotrexate. Cyclophosphamide and methotrexate applied topically produced no systemic side effects, and only one mild and transient local side effect in the form of hyperemia of the bulbar and palpebral conjunctiva. These two immunosuppressive drugs were more effective than the corticosteroid currently in clinical use, i.e., dexamethasone.

摘要

通过对受体兔眼局部应用环磷酰胺（0.1%于无菌等渗氯化钠溶液中）和甲氨蝶呤（0.1%于花生油中），每日4次，持续4周，兔角膜移植的存活时间显著延长。一组对照组动物以同样方式用 dexamethasone 治疗。用 dexamethasone 治疗的组平均移植存活时间为52±18.5天，用环磷酰胺和甲氨蝶呤治疗的组分别为80±18.2天和73±23.5天。局部应用环磷酰胺和甲氨蝶呤未产生全身副作用，仅出现一例轻度、短暂的局部副作用，表现为眼球结膜和睑结膜充血。这两种免疫抑制药物比目前临床使用的皮质类固醇，即 dexamethasone，更有效。