Gasić S, Bratusch-Marrain P, Waldhäusl W, Korn A
First Medical University Clinic, Department of Clinical Pharmacology, Vienna, Austria.
Int J Clin Pharmacol Ther Toxicol. 1988 Jan;26(1):36-40.
To determine the effect of verapamil on basal and glucagon-induced changes in splanchnic glucose metabolism and insulin secretion, six healthy men were studied during a primed-continuous infusion of verapamil (2.5 micrograms/kg/min). After a basal period of 30 min, glucagon was infused at a rate of 12 ng/kg/min for an additional 60 min. Splanchnic exchange of glucose, lactate, alanine and beta-hydroxy-butyrate, as well as splanchnic C-peptide release, reflecting insulin production rate, were determined by means of the liver vein catheter technique. Intraindividual control trials without verapamil were performed. Basal and glucagon-stimulated arterial glucose and insulin concentrations as well as insulin production rate were not significantly altered by verapamil, which also had no influence on basal splanchnic glucose output or on the glucagon-induced initial (0-30 min) increment in splanchnic glucose output. However, the gradual decline in splanchnic glucose output was delayed at 30-60 min (verapamil: 55.85 +/- 6.31 control: 39.73 +/- 5.6 mmol/30 min; mmol/30 min, mean, s.e.m., p less than 0.02). Splanchnic lactate-alanine- and beta-hydroxy-butyrate exchange were also unchanged by verapamil. We conclude that verapamil attenuates glucagon's evanescent effect on splanchnic glucose output without interfering with the exchange of gluconeogenic precursors or beta-hydroxy-butyrate or insulin production rate.
为了确定维拉帕米对基础状态下以及胰高血糖素诱导的内脏葡萄糖代谢和胰岛素分泌变化的影响，对6名健康男性在持续输注维拉帕米（2.5微克/千克/分钟）并给予负荷剂量的情况下进行了研究。在30分钟的基础期后，以12纳克/千克/分钟的速率输注胰高血糖素，持续60分钟。通过肝静脉导管技术测定葡萄糖、乳酸、丙氨酸和β-羟基丁酸的内脏交换，以及反映胰岛素生成率的内脏C肽释放。进行了无维拉帕米的个体内对照试验。维拉帕米对基础状态和胰高血糖素刺激后的动脉葡萄糖和胰岛素浓度以及胰岛素生成率没有显著影响，对基础内脏葡萄糖输出或胰高血糖素诱导的内脏葡萄糖输出初始（0 - 30分钟）增加也没有影响。然而，在30 - 60分钟时，内脏葡萄糖输出的逐渐下降被延迟（维拉帕米组：55.85±6.31；对照组：39.73±5.6毫摩尔/30分钟；毫摩尔/30分钟，均值，标准误，p<0.02）。维拉帕米对内脏乳酸 - 丙氨酸和β - 羟基丁酸交换也没有影响。我们得出结论，维拉帕米减弱了胰高血糖素对内脏葡萄糖输出的短暂影响，而不干扰糖异生前体或β - 羟基丁酸的交换或胰岛素生成率。
