Department of Molecular Medicine and Surgery, Karolinska Institute, Stockholm, Sweden.
Department of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
J Ultrasound Med. 2019 Jul;38(7):1723-1731. doi: 10.1002/jum.14858. Epub 2018 Nov 13.
Ultrasound biomicroscopy (UBM), or ultra high-frequency ultrasound, is a technique used to assess the anatomy of small research animals. In this study, UBM was used to assess differences in intimal hyperplasia thickness as a surrogate measurement of the re-endothelialization process after carotid artery balloon injury in rats.
Ultrasound biomicroscopic data from 3 different experiments and rat strains (Sprague Dawley, Wistar, and diabetic Goto-Kakizaki) were analyzed. All animals were subjected to carotid artery balloon injury and examined with UBM (30-70 MHz) 2 and 4 weeks after injury. Re-endothelialization on UBM was defined as the length from the carotid bifurcation to the most distal visible edge of the intimal hyperplasia. En face staining with Evans blue dye was performed at euthanasia 4 weeks after injury, followed by tissue harvesting for histochemical and immunohistochemical evaluations.
A significant correlation (Spearman r = 0.63; P < .0001) was identified when comparing all measurements of re-endothelialization obtained from UBM and en face staining. The findings revealed a similar pattern for all rat strains: Sprague Dawley (Spearman r = 0.70; P < .0001), Wistar (Spearman r = 0.36; P < .081), and Goto-Kakizaki (Spearman r = 0.70; P < .05). A Bland-Altman test showed agreement between en face staining and UBM. Immunohistochemical staining confirmed the presence of the endothelium in the areas detected as re-endothelialized by the UBM assessment.
Ultrasound biomicroscopy can be used for repeated in vivo assessment of re-endothelialization after carotid artery balloon injury in rats.
超声生物显微镜（UBM）或超高频率超声是一种用于评估小型研究动物解剖结构的技术。在本研究中，UBM 用于评估颈动脉球囊损伤后大鼠内膜增生厚度的差异，作为再内皮化过程的替代测量指标。
分析了 3 项不同实验和大鼠品系（Sprague Dawley、Wistar 和糖尿病 Goto-Kakizaki）的超声生物显微镜数据。所有动物均接受颈动脉球囊损伤，并在损伤后 2 周和 4 周进行 UBM（30-70 MHz）检查。UBM 上的再内皮化定义为颈动脉分叉处至内膜增生最远端可见边缘的长度。损伤后 4 周行安乐死时进行 Evans 蓝染料的面染色，随后进行组织学和免疫组织化学评估。
UBM 和面染色的再内皮化所有测量值之间存在显著相关性（Spearman r=0.63；P<0.0001）。所有大鼠品系均显示出相似的模式：Sprague Dawley（Spearman r=0.70；P<0.0001）、Wistar（Spearman r=0.36；P<0.081）和 Goto-Kakizaki（Spearman r=0.70；P<0.05）。Bland-Altman 检验显示面染色与 UBM 之间具有一致性。免疫组织化学染色证实了在 UBM 评估中检测到的再内皮化区域存在内皮细胞。
超声生物显微镜可用于重复评估大鼠颈动脉球囊损伤后再内皮化的体内情况。
