Department of Obstetrics and Gynecology, Faculty of Medicine, Kagoshima University, Kagoshima, Japan,
Department of Obstetrics and Gynecology, Faculty of Medicine, Kagoshima University, Kagoshima, Japan.
Oncology. 2019;96(4):179-182. doi: 10.1159/000493485. Epub 2018 Nov 14.
The detection accuracy of sentinel lymph node (SLN) metastasis by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) for endometrial cancer (EC) remains unclear and was assessed in this preliminary study.
We studied primary cancer tissues and pelvic lymph nodes (PLN) from 105 patients with EC. qRT-PCR assay was performed to determine the copy numbers of CK19 mRNA in EC tissues, and negative and positive LN samples. Further, qRT-PCR results were compared with pathological findings.
CK19 mRNA expression was detected in 98% (104/106) of the tumors, with a median copy number of 3.0 × 105/μL. Twelve LN were diagnosed as positive by pathological examination. The median copy number of CK19 mRNA for positive and negative LN was 8.1 × 104/μL and 90.4/µL, respectively. CK19 mRNA expression was higher in pathologically positive LN than in pathologically negative LN (p < 0.01); the pathological and qRT-PCR findings showed no discrepancy. When the cutoff value was set at 4,500 copies/µL, qRT-PCR assay using CK19 mRNA exhibited high sensitivity and specificity.
Our results demonstrated that qRT-PCR assay, using CK19 mRNA, exhibits a high accuracy for detecting LN metastasis in EC and represents a useful alternative to conventional pathological diagnosis of EC.
定量逆转录聚合酶链反应（qRT-PCR）检测子宫内膜癌（EC）前哨淋巴结（SLN）转移的准确性尚不清楚，本初步研究对此进行了评估。
我们研究了 105 例 EC 患者的原发癌组织和盆腔淋巴结（PLN）。采用 qRT-PCR 法测定 EC 组织、阴性和阳性 LN 样本中 CK19 mRNA 的拷贝数。进一步将 qRT-PCR 结果与病理发现进行比较。
98%（104/106）的肿瘤中检测到 CK19 mRNA 表达，中位拷贝数为 3.0×105/μL。12 个 LN 通过病理检查诊断为阳性。CK19 mRNA 阳性和阴性 LN 的中位拷贝数分别为 8.1×104/μL 和 90.4/μL。CK19 mRNA 表达在病理阳性 LN 中高于病理阴性 LN（p<0.01）；病理和 qRT-PCR 结果无差异。当截取值设定为 4500 拷贝/μL 时，CK19 mRNA 的 qRT-PCR 检测显示出较高的灵敏度和特异性。
我们的结果表明，CK19 mRNA 的 qRT-PCR 检测对 EC 中 LN 转移具有较高的准确性，是 EC 传统病理诊断的有用替代方法。
