Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun, Jilin, China (mainland).
People's Hospital of Jilin Province, Changchun, Jilin, China (mainland).
Med Sci Monit. 2018 Nov 14;24:8198-8206. doi: 10.12659/MSM.911175.
BACKGROUND Hydrogen peroxide-induced neuronal oxidative stress is a serious threat to the nervous system. Catechins and related compounds are effective radical scavengers that protect against nerve cell damage. MATERIAL AND METHODS Here, we investigated the antioxidant property of various catechins in protecting against hydrogen peroxide, as well as their radical-scavenging activity. RESULTS We found that catechins treatment effectively protected HT22 cells against H₂O₂-induced cell viability by decreasing and attenuating reactive oxidative species production in different proportions. In addition, all tested catechins performed radical scavenging activity, and partially removed the free radicals. Among all investigated catechins, epigallocatechin gallate was the most effective against ROS production and had the strongest radical-scavenging activity. These results suggest that beneficial effects were strongly related with structure of catechins, mainly because of the hydroxyl and galloyl groups. CONCLUSIONS In conclusion, epigallocatechin gallate is the most effective antioxidant polyphenol against hydrogen peroxide and radical-scavenging activity.
过氧化氢诱导的神经元氧化应激对神经系统是一个严重的威胁。儿茶素和相关化合物是有效的自由基清除剂，可防止神经细胞损伤。
在这里，我们研究了各种儿茶素在抵御过氧化氢以及它们的自由基清除活性方面的抗氧化特性。
我们发现儿茶素处理通过以不同比例减少和减弱活性氧的产生，有效地保护 HT22 细胞免受 H₂O₂诱导的细胞活力下降。此外，所有测试的儿茶素都具有自由基清除活性，并部分去除自由基。在所有研究的儿茶素中，没食子儿茶素没食子酸酯对 ROS 产生的抑制作用最强，自由基清除活性最强。这些结果表明，有益的作用与儿茶素的结构密切相关，主要是因为其羟基和没食子酰基。
总之，没食子儿茶素没食子酸酯是最有效的抗氧化多酚，可对抗过氧化氢和自由基清除活性。
