基于与黄曲霉毒素B₁、B₂、G₁和G₂反应的单克隆抗体开发用于分析总黄曲霉毒素的酶联免疫吸附测定法。
Development of Enzyme-Linked Immunosorbent Assay for Analysis of Total Aflatoxins Based on Monoclonal Antibody Reactive with Aflatoxins B, B, G and G.
作者信息
Yamasaki Tomomi, Miyake Shiro, Sato Natsuki, Hirakawa Yuki, Iwasa Seiji, Narita Hiroshi, Watanabe Takaho
机构信息
Kyoto Women's University.
Advanced Science, Technology & Management Research Institute of Kyoto.
出版信息
Shokuhin Eiseigaku Zasshi. 2018;59(5):200-205. doi: 10.3358/shokueishi.59.200.
A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was developed for the determination of total amount of aflatoxin B, B, G and G (AFB, AFB, AFG and AFG), using a mouse monoclonal antibody that shows similar reactivity to each of these AFs. The working range of the developed dc-ELISA was 50-230 pg/mL for AFB, 50-270 pg/mL for AFB, 60-390 pg/mL for AFG and 65-700 pg/mL for AFG. The recovery of AFs from spiked roasted peanuts was 98％. Further, when 4 samples actually contaminated with AFB, AFB, AFG and AFG were examined, the results of dc-ELISA were highly correlated with the values assigned by the Food Analysis Performance Assessment Scheme. The developed dc-ELISA appears to be suitable for the determination of total AFs at concentrations around the maximum permitted level (10 μg/kg for all foods) in Japan.
开发了一种直接竞争酶联免疫吸附测定法（dc-ELISA），用于测定黄曲霉毒素B₁、B₂、G₁和G₂（AFB₁、AFB₂、AFG₁和AFG₂）的总量，该方法使用对上述每种黄曲霉毒素均具有相似反应性的小鼠单克隆抗体。所开发的dc-ELISA的工作范围为：AFB₁为50 - 230 pg/mL，AFB₂为50 - 270 pg/mL，AFG₁为60 - 390 pg/mL，AFG₂为65 - 700 pg/mL。加标烤花生中黄曲霉毒素的回收率为98％。此外，当检测4份实际被AFB₁、AFB₂、AFG₁和AFG₂污染的样品时，dc-ELISA的结果与食品分析性能评估计划指定的值高度相关。所开发的dc-ELISA似乎适用于测定日本最大允许含量（所有食品均为10 μg/kg）左右浓度的总黄曲霉毒素。
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