Effects of alcohol on ganglion cell receptive field properties and sensitivity in the frog retina.

Previous results have shown that alcohol has an effect on vision and on the excitability of retinal neurons. Action potentials of single ganglion cells were recorded by microelectrodes in opened and excised eyes from frogs (Rana temporaria L.). Histologically two types of synapses have been described in the retina: conventional synapses and synapses with a ribbon or bar shaped component surrounded by a rather uniform layer of synaptic vesicles. The "ribbon synapses" are presynaptic contacts in receptor and bipolar cells while horizontal and amacrine cells have conventional synapses. Tests with ethanol doses up to 0.2% indicated stronger effects on the conventional synapses than on the ribbon synapses. Alcohol decreased or abolished the lateral inhibition ( inhibitory surround) mediated by the amacrine cells and depressed the signals from the green rods, which apparently are mediated by horizontal cells. Further alcohol decreased the sensitivity of the signals from the completely dark-adapted red rods in the retina, and increased the sensitivity of the cone-mediated responses for class 3 and deviating class 4 cells, when measured against a background light. Alcohol also increased the latency of the response up to 55 msec. depending on the size of the stimulus field.