Vidotto V, Picerno G, Caramello S, Paniate G
Istituto di Malattie Infettive, Facoltà di Medicina, Università degli Studi di Torino, Italy.
Microbiologica. 1988 Apr;11(2):143-50.
Growth of Saccharomyces cerevisiae strain Castelli 20 has been observed in a minimal synthetic medium using different glucose concentrations and without biotin, during the first 30 hrs of its development at 28 degrees C. The yeast's growth was observed spectrophotometrically at 675 nm reading the Optical Density every hour. The minimal medium of Sheperd et al. (1980), with glucose 15 g/L and biotin was modified: the vitamin was eliminated and the concentration of glucose was gradually reduced down to 0.5 g/L. At 5 g/L of glucose concentration and without vitamins the strain grew very well. From our results up to 30 hrs biotin has no influence on the yeast's growth. The medium would be useful to study Saccharomyces cerevisiae physiology during the early period of its development.
在28摄氏度下，酿酒酵母Castelli 20菌株在最初30小时的发育过程中，于不含生物素的不同葡萄糖浓度的合成基本培养基中进行了生长观察。通过分光光度法在675nm处每小时读取光密度来观察酵母的生长情况。Sheperd等人（1980年）的基本培养基，含有15g/L葡萄糖和生物素，进行了改良：去除了维生素，并将葡萄糖浓度逐渐降低至0.5g/L。在5g/L葡萄糖浓度且无维生素的情况下，该菌株生长良好。根据我们的结果，在长达30小时的时间里，生物素对酵母生长没有影响。该培养基对于研究酿酒酵母发育早期的生理学很有用。
