Wei Pingying, Li Zilong, Lin Yuping, He Peng, Jiang Ning
Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100080, P.R. China.
Biotechnol Lett. 2007 Oct;29(10):1501-8. doi: 10.1007/s10529-007-9419-1. Epub 2007 May 31.
An effective, simple, and convenient method to improve yeast's multiple-stress tolerance, and ethanol production was developed. After an ethanologenic Saccharomyces cerevisiae strain SC521 was treated by nine cycles of freeze-thaw, a mutant FT9-11 strain with higher multiple-stress tolerance was isolated, whose viabilities under acetic acid, ethanol, freeze-thaw, H(2)O(2), and heat-shock stresses were, respectively, 23-, 26-, 10- and 7-fold more than the parent strain at an initial value 2 x 10(7) c.f.u. per ml. Ethanol production of FT9-11 was similar (91.5 g ethanol l(-1)) to SC521 at 30 degrees C with 200 g glucose l(-1), and was better than the parent strain at 37 degrees C (72.5 g ethanol l(-1)), with 300 (111 g ethanol l(-1)) or with 400 (85 g ethanol l(-1)) g glucose l(-1).
开发了一种有效、简单且便捷的方法来提高酵母的多重胁迫耐受性及乙醇产量。将产乙醇的酿酒酵母菌株SC521进行9次冻融处理后，分离得到了具有更高多重胁迫耐受性的突变体FT9-11菌株，在初始值为每毫升2×10⁷ c.f.u.时，其在乙酸、乙醇、冻融、H₂O₂和热激胁迫下的存活率分别比亲本菌株高23倍、26倍、10倍和7倍。在30℃、200 g葡萄糖/升的条件下，FT9-11的乙醇产量（91.5 g乙醇/升）与SC521相似，在37℃（72.5 g乙醇/升）、300 g葡萄糖/升（111 g乙醇/升）或400 g葡萄糖/升（85 g乙醇/升）的条件下，FT9-11的乙醇产量优于亲本菌株。
