Kim Y C, Kudo H, Ogawa K, Ohshio G, Aye T T, Nakashima Y, Takakura K, Fujii S, Inada M, Yamabe H
Department of Pathology, Faculty of Medicine, Kyoto University, Japan.
Am J Obstet Gynecol. 1988 Aug;159(2):522-6. doi: 10.1016/s0002-9378(88)80121-2.
The localization of vitamin B12 R-binder in the uterus was studied by use of an immunoperoxidase technique. Positive staining by anti-R-binder antiserum was observed in the columnar epithelium of the endocervix (18/18 cases) and in the surface epithelium of the endometrium (8/21 cases). Staining was usually seen in the apical portion of the epithelium; cytoplasmic staining in the endocervical columnar epithelium was intense. The secretory products in the endocervical glands showed positive staining. The endometrial glandular epithelium did not stain (0/24 cases). Metaplastic squamous epithelium of the endocervix showed positive staining (3/18 cases). The native squamous epithelium as well as the stromal components of the cervix, endometrium, and myometrium were negative for R-binder. This study shows that R-binder is localized in the uterus, especially in the endocervical glands. The R-binder in the endocervix may have antimicrobial activity in the uterus as in other organs, such as the intestines and mammary glands.
采用免疫过氧化物酶技术研究了子宫中维生素B12 R结合蛋白的定位。在宫颈柱状上皮（18/18例）和子宫内膜表面上皮（8/21例）观察到抗R结合蛋白抗血清的阳性染色。染色通常见于上皮的顶端部分；宫颈柱状上皮中的细胞质染色强烈。宫颈腺中的分泌产物呈阳性染色。子宫内膜腺上皮未染色（0/24例）。宫颈化生鳞状上皮呈阳性染色（3/18例）。宫颈、子宫内膜和子宫肌层的天然鳞状上皮以及基质成分R结合蛋白均为阴性。本研究表明，R结合蛋白定位于子宫，尤其是宫颈腺。宫颈中的R结合蛋白可能像在肠道和乳腺等其他器官一样，在子宫中具有抗菌活性。
