Van Scott M R, Lee N P, Yankaskas J R, Boucher R C
Department of Medicine, University of North Carolina, Chapel Hill 27599-7020.
Am J Physiol. 1988 Aug;255(2 Pt 1):C237-45. doi: 10.1152/ajpcell.1988.255.2.C237.
Insulin (INS), endothelial cell growth supplement (ECGS), transferrin (TF), cholera toxin (CT), hydrocortisone (HC), triiodothyronine (T3), and epidermal growth factor (EGF) were systematically examined for their effects on proliferation, ion transport activity, and morphological differentiation of canine tracheal epithelial (CTE) cells in culture. INS, ECGS, TF, and CT increase proliferation of CTE cells cultured on plastic Petri dishes but exhibit no acute effect on the bioelectric activity of freshly excised CTE. CT increases amiloride-insensitive transepithelial ion transport across both freshly excised canine trachea and CTE cultures. EGF has no effect on proliferation of CTE cells on plastic but induces hyperplasia of CTE cells on collagen matrices. EGF does not alter basal transepithelial ion transport across cultures but increases cellular responsiveness to beta-adrenergic stimulation. HC and T3 have no effect on proliferation or transepithelial bioelectric properties of CTE cells but improve morphological differentiation yielding cultures of complex epithelia containing cuboidal ciliated and nonciliated cells. These results demonstrate that growth and function of cultured CTE cells are affected by specific growth factors.
对胰岛素（INS）、内皮细胞生长补充剂（ECGS）、转铁蛋白（TF）、霍乱毒素（CT）、氢化可的松（HC）、三碘甲状腺原氨酸（T3）和表皮生长因子（EGF）对培养的犬气管上皮（CTE）细胞增殖、离子转运活性和形态分化的影响进行了系统研究。INS、ECGS、TF和CT可增加在塑料培养皿中培养的CTE细胞的增殖，但对新鲜分离的CTE的生物电活性无急性影响。CT可增加经新鲜分离的犬气管和CTE培养物的氨氯地平不敏感的跨上皮离子转运。EGF对塑料培养皿上的CTE细胞增殖无影响，但可诱导胶原基质上的CTE细胞增生。EGF不会改变跨培养物的基础跨上皮离子转运，但会增加细胞对β-肾上腺素能刺激的反应性。HC和T3对CTE细胞的增殖或跨上皮生物电特性无影响，但可改善形态分化，产生含有立方纤毛和非纤毛细胞的复杂上皮培养物。这些结果表明，培养的CTE细胞的生长和功能受特定生长因子的影响。
