Chopra D P, Siddiqui K M, Cooney R A
Gastroenterology. 1987 Apr;92(4):891-904. doi: 10.1016/0016-5085(87)90962-0.
Primary and serially passaged human fetal normal colon epithelial cells have been propagated and characterized with regard to their nature and origin. The cells exhibited many characteristics of colonic epithelial cells including the presence of mucopolysaccharides and carcinoembryonic antigen. Serial passaging of the cultures required supplementing the medium with insulin, transferrin, epidermal growth factor, and cholera toxin. This study also shows that these factors have specific roles in the regulation of growth and morphologic differentiation of the cell cultures. Insulin apparently is mainly associated with cell multiplication, whereas transferrin, epidermal growth factor, and cholera toxin are associated with the maintenance of morphologic differentiation status of the cell cultures.
原代及传代培养的人胎儿正常结肠上皮细胞已得到增殖，并就其性质和来源进行了鉴定。这些细胞表现出结肠上皮细胞的许多特征，包括存在粘多糖和癌胚抗原。对培养物进行传代培养需要在培养基中补充胰岛素、转铁蛋白、表皮生长因子和霍乱毒素。本研究还表明，这些因子在细胞培养物的生长调节和形态分化中具有特定作用。胰岛素显然主要与细胞增殖有关，而转铁蛋白、表皮生长因子和霍乱毒素则与维持细胞培养物的形态分化状态有关。
