Baek Hongchae, Sariev Anvar, Kim Min-Ju, Lee Hojin, Kim Jinhyun, Kim Hyungmin
Annu Int Conf IEEE Eng Med Biol Soc. 2018 Jul;2018:4744-4747. doi: 10.1109/EMBC.2018.8513138.
The role of established contralateral cerebrocerebellar connections on cerebellar injury during stroke has been increasingly revealed in recent years. An extensive number of studies have investigated alteration in inter-hemispheric correlation in order to find brain regions whose responses are specific to restore functional loss and enhance adaptive neural plasticity after stroke. Although, several non-invasive brain stimulation studies have proven their efficacy in the treatment of stroke recovery, finding more effective brain regions that responsible for stroke rehabilitation as well as optimizing neural stimulation protocol are the main goals of further investigations. In this study, the lateral cerebellar nucleus (LCN) was exposed to Low-Intensity Focused Ultrasound (LIFU) to reduce the cerebellar damage resulting from crossed cerebellar diaschisis (CCD) phenomenon after cerebral ischemia. A mouse brain ischemia was induced by middle cerebral artery occlusion (MCAO). A level of decrease in Purkinje cell (PC) number and a quantity of myeloperoxidase (MPO) positive neutrophils in the cerebral cortex were compared between stroke and stroke+LIFU groups after MCAO. In stroke+LIFU group, the increased ipsilateral water content due to tissue swelling was observed, showing an attenuation of brain edema. Prominently, the reduction of the neuroimmune reactivity at the infarct core and the peri-infarct regions, and the increased rate of survival among PCs clearly demonstrated primary evidence of neuroprotective effect induced by LIFU-mediated cerebellar modulation.
近年来，已建立的对侧脑桥小脑连接在中风时小脑损伤中的作用日益显现。大量研究调查了半球间相关性的改变，以寻找那些对恢复功能丧失和增强中风后适应性神经可塑性具有特异性反应的脑区。尽管一些非侵入性脑刺激研究已证明其在中风恢复治疗中的疗效，但找到更多负责中风康复的有效脑区以及优化神经刺激方案是进一步研究的主要目标。在本研究中，将低强度聚焦超声（LIFU）作用于外侧小脑核（LCN），以减轻脑缺血后交叉小脑失联络（CCD）现象导致的小脑损伤。通过大脑中动脉闭塞（MCAO）诱导小鼠脑缺血。比较MCAO后中风组和中风+LIFU组之间浦肯野细胞（PC）数量的减少水平以及大脑皮质中髓过氧化物酶（MPO）阳性中性粒细胞的数量。在中风+LIFU组中，观察到由于组织肿胀导致同侧含水量增加，表明脑水肿有所减轻。显著的是，梗死核心区和梗死周围区域神经免疫反应性的降低以及PCs存活率的提高清楚地证明了LIFU介导的小脑调节诱导神经保护作用的主要证据。
