de Jesus Pires de Moraes Alan, Andreato Leonardo Vidal, Branco Braulio Henrique Magnani, da Silva Edson Luiz, Gonçalves Márcia Aparecida, Dos Santos Rafaella Zulianello, Becker Aline Minuzzi, da Silveira Cavalcante Luciana, da Silva Casagrande Fernanda, Benetti Magnus
Sciences Center of Health and Sport, State University of Santa Catarina, Florianópolis, Brazil.
University of the Itajaí Valley, Itajaí, Brazil.
J Exerc Rehabil. 2018 Oct 31;14(5):802-809. doi: 10.12965/jer.1836152.076. eCollection 2018 Oct.
The purpose of this study was to evaluate the effects of N-acetylcysteine (NAC) supplementation on cellular damage and oxidative stress indicators in volleyball athletes. Twenty male volleyball athletes at national level performed a physical training session and were divided into 2 groups, which for 7 days took the placebo substance or NAC. After 7 days the athletes repeated the same training session. In both sessions, blood samples were collected 30 min before and immediately after the training session to measure cellular damage and oxidative stress markers. The main results show that, although higher concentrations of glutathione peroxidase and superoxide dismutase were observed in post-session 1 than those in postsession 2, the other markers showed an increase in antioxidant action after supplementation of NAC, once the effect of experimental conditions (=0.030) were observed in: time effect (<0.001) and interaction (=0.019) for total glutathione; time effect (<0.001) and interaction (<0.001) for reduced glutathione; and time effect (<0.001) for ferric-reducing antioxidant potential. The oxidant action indicated by the protein carbonyl was higher in the placebo group than in the NAC group (=0.028), but a time effect (<0.001) for the thiobarbituric acid reactive substances showed lower values in presession 1 than in presession 2. For the cellular damage markers, antagonistic results between markers were found. Based in the results, the supplementation of NAC during a short period was effective in reducing oxidant action and increasing antioxidant action. However, conclusive alterations in the responses of the cellular damage markers were not obtained.
本研究的目的是评估补充N-乙酰半胱氨酸（NAC）对排球运动员细胞损伤和氧化应激指标的影响。20名国家级男性排球运动员进行了一次体能训练，并被分为两组，连续7天分别服用安慰剂或NAC。7天后，运动员重复相同的训练课程。在这两次训练中，分别在训练前30分钟和训练结束后立即采集血样，以测量细胞损伤和氧化应激标志物。主要结果表明，虽然在训练后1中观察到的谷胱甘肽过氧化物酶和超氧化物歧化酶浓度高于训练后2，但在补充NAC后，其他标志物的抗氧化作用有所增加，因为在以下方面观察到了实验条件的影响（=0.030）：总谷胱甘肽的时间效应（<0.001）和交互作用（=0.019）；还原型谷胱甘肽的时间效应（<0.001）和交互作用（<0.001）；以及铁还原抗氧化能力的时间效应（<0.001）。安慰剂组中蛋白质羰基所指示的氧化作用高于NAC组（=0.028），但硫代巴比妥酸反应性物质的时间效应（<0.001）表明，训练前1的值低于训练前2。对于细胞损伤标志物，各标志物之间存在拮抗结果。基于这些结果，短期内补充NAC可有效降低氧化作用并增加抗氧化作用。然而，未获得细胞损伤标志物反应的确切变化。
