侵袭性伪足检测与明胶降解试验。
Invadopodia Detection and Gelatin Degradation Assay.
作者信息
Díaz Begoña
机构信息
Signal Transduction Program, Sanford Burnham Medical Research Institute, La Jolla, CA, USA.
出版信息
Bio Protoc. 2013 Dec 20;3(24). doi: 10.21769/BioProtoc.997.
Abstract
This protocol is designed to quantify invadopodia formation and activity. Invadopodia are protrusive structures elaborated by cancer cells that mediate cell attachment and remodeling of the extracellular matrix. These structures contribute to the ability of cancer cells to invade and metastasize. In this protocol, both the presence of invadopodia and their activity is simultaneously assessed and quantified by a fluorescent microscopy-based assay.
摘要
本方案旨在量化侵袭性伪足的形成和活性。侵袭性伪足是癌细胞形成的突出结构，介导细胞附着和细胞外基质重塑。这些结构有助于癌细胞的侵袭和转移能力。在本方案中，通过基于荧光显微镜的检测方法同时评估和量化侵袭性伪足的存在及其活性。
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