Pradines-Figueres A, Vannier C, Ailhaud G
Laboratoire de Biologie du Développement du Tissu Adipeux, Centre de Biochimie du CNRS, Faculté des Sciences, Nice, France.
Biochem Biophys Res Commun. 1988 Aug 15;154(3):982-90. doi: 10.1016/0006-291x(88)90236-7.
The spontaneous secretion of lipoprotein lipase has been examined in adipose cells of mouse Ob17, Ob17SA and 3T3-F442A clonal lines as well as in rat adipose cells in primary culture. Striking differences are observed both in serum-free and serum-supplemented media, rat adipose cells and 3T3-F442A cells being the most active. Insulin from 10(-11) M to 10(-9) M was able to modulate the rate of LPL secretion from 2- to 4-fold. The stimulatory effect of insulin on this process occurred within 30 min in cells treated or not with cycloheximide. It is concluded that insulin is able to modulate the rate of LPL secretion independently of the synthesis of new enzyme molecules on a short-term basis and within a physiological range of concentrations.
在小鼠Ob17、Ob17SA和3T3-F442A克隆系的脂肪细胞以及原代培养的大鼠脂肪细胞中，对脂蛋白脂肪酶的自发分泌进行了检测。在无血清和补充血清的培养基中均观察到显著差异，大鼠脂肪细胞和3T3-F442A细胞最为活跃。浓度从10(-11)M至10(-9)M的胰岛素能够将脂蛋白脂肪酶的分泌速率调节2至4倍。在经或未经环己酰亚胺处理的细胞中，胰岛素对这一过程的刺激作用在30分钟内即可出现。得出的结论是，胰岛素能够在短期内且在生理浓度范围内独立于新酶分子的合成来调节脂蛋白脂肪酶的分泌速率。
