van de Wijngaert F P, Schipper C A, Tas M C, Burger E H
Department of Oral Cell Biology, Academic Center of Dentistry, ACTA, Amsterdam, The Netherlands.
Bone. 1988;9(2):81-8. doi: 10.1016/8756-3282(88)90107-x.
Periost-free, live and/or devitalized cartilaginous long bone rudiments of fetal mice were transplanted under the renal capsule of adult syngeneic mice to study the role of cells and intercellular matrix in the recruitment and formation of osteoclasts and osteoblasts, both identified by means of enzyme- and immunohistochemical methods. Live bone rudiments recruited host-derived osteoclasts within 5 days after transplantation. Osteoblasts developed as rapidly as osteoclasts and participated in the modeling of the rudiments into hemopoietic bone marrow containing ossicles. Devitalized bone rudiments, killed before osteoclastic invasion had occurred, did not recruit osteoclasts or osteoblasts, and were not resorbed up till 35 days after transplantation. Co-transplantation of live and devitalized bone rudiments however resulted in osteoclastic resorption of the killed rudiments, starting 9 days after transplantation. Again the live rudiments were modeled into ossicles. Devitalized bone rudiments which had been invaded by osteoclasts before killing and transplantation, did recruit host osteoclasts, but at a slower rate than live rudiments, and depending on the number of resorption sites at the time of transplantation. Osteoblasts were not formed. These data suggest that in developing long bones chondrocyte activity is involved in the recruitment of osteoclasts as well as osteoblasts. Matrix components diffusing from resorbing surfaces seem to be involved in osteoclast recruitment.
将无骨膜、活的和/或失活的胎鼠软骨性长骨原基移植到同基因成年小鼠的肾被膜下，以研究细胞和细胞间基质在破骨细胞和成骨细胞募集及形成中的作用，这两种细胞均通过酶法和免疫组化方法鉴定。活的骨原基在移植后5天内募集宿主来源的破骨细胞。成骨细胞与破骨细胞发育速度一样快，并参与将原基塑造成含有小骨的造血骨髓。在破骨细胞侵入之前就已失活的骨原基，在移植后35天内都不会募集破骨细胞或成骨细胞，也不会被吸收。然而，将活的和失活的骨原基共同移植，会导致失活的原基在移植后9天开始出现破骨细胞吸收。同样，活的原基会被塑造成小骨。在失活和移植前已被破骨细胞侵入的失活骨原基，确实会募集宿主破骨细胞，但速度比活的原基慢，且取决于移植时吸收位点的数量。不会形成成骨细胞。这些数据表明，在发育中的长骨中，软骨细胞活性参与了破骨细胞和成骨细胞的募集。从吸收表面扩散的基质成分似乎参与了破骨细胞的募集。
