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广西木兰叶多酚的提取及其抗氧化活性

Extraction and Antioxidant Activities of Magnolia kwangsiensis Figlar & Noot. Leaf Polyphenols.

作者信息

Zheng Yan-Fei, Liu Xiong-Min, Lai Fang

机构信息

School of Chemistry and Chemical Engineering, Guangxi Normal, University for Nationalities, Chongzuo, 532200, P. R. China.

School of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, P. R. China.

出版信息

Chem Biodivers. 2019 Feb;16(2):e1800409. doi: 10.1002/cbdv.201800409. Epub 2019 Jan 23.

Abstract

Microwave-assisted extraction was employed to extract polyphenols from the leaf of Magnolia kwangsiensis Figlar & Noot. The yield of polyphenols was 2.44±0.02 % under the optimal conditions of RSM: acetone concentration of 70 %, ratio of solvent to material of 21 mL⋅g and extraction time of 16 min. The antioxidant activities were evaluated in terms of total antioxidant ability, reducing power, DPPH and OH scavenging activity. Results showed the polyphenols presented potential antioxidant activities, especially the stronger scavenging activity on OH. In term of OH scavenging activity, the IC value of NKA-9 purification was 0.335 mg mL , equivalent to 35.23 % of V . The IC values of crude extract and ethyl acetate extract were 0.580 and 0.828 mg mL , equivalent to 60.99 % and 87.07 % of V . Results indicated that M. kwangsiensis leaf polyphenols present potential antioxidant activities that make it beneficial for human health by preventing or reducing oxidative damage.

摘要

采用微波辅助萃取法从广西木兰(Magnolia kwangsiensis Figlar & Noot.)叶片中提取多酚。在响应面法优化的条件下:丙酮浓度70%、料液比21 mL·g、提取时间16 min,多酚得率为2.44±0.02%。从总抗氧化能力、还原力、DPPH和·OH清除活性方面对其抗氧化活性进行评价。结果表明,多酚具有潜在的抗氧化活性,尤其是对·OH具有较强的清除活性。在·OH清除活性方面,NKA-9纯化产物的IC值为0.335 mg·mL,相当于维生素C(Vc)的35.23%。粗提物和乙酸乙酯提取物的IC值分别为0.580和0.828 mg·mL,相当于Vc的60.99%和87.07%。结果表明,广西木兰叶片多酚具有潜在的抗氧化活性,通过预防或减少氧化损伤对人体健康有益。

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