Citrus Research Institute, Southwest University, Chongqing, 400712, P. R. China, and Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China.
Plant Dis. 2019 Jan;103(1):65-68. doi: 10.1094/PDIS-05-18-0911-RE. Epub 2018 Nov 16.
In 2009, a new citrus viral disease caused by Citrus yellow vein clearing virus (CYVCV) was first discovered in China and now CYVCV is widely distributed in the field. CYVCV is transmissible by grafting and is spread by aphids from lemon to bean, and from bean to bean. However, until now, no vector has been shown to transmit CYVCV from citrus to citrus. In this study, after a 24-h acquisition access period (AAP), CYVCV was tested for in Dialeurodes citri (Ashmead), Panonychus citri McGregor, and Aphis citricidus (Kirkaldy) by quantitative RT-PCR. After an AAP of 48 h, groups of adults of D. citri, P. citri, and A. citricidus were given a 48 h inoculation access period on cultivar Daidai sour orange seedlings. Three, 6, and 12 months post-transmission by D. citri, CYVCV was detected in the receptor plants, and the mean incidence of infected trees was 31.9, 39.1, and 39.1%, respectively. CYVCV was not transmitted to citrus by P. citri or A. citricidus. This is the first report of the ability of D. citri to transmit CYVCV from infected to healthy citrus under laboratory conditions.
2009 年，中国首次发现由柑橘黄龙病清毒病毒（CYVCV）引起的一种新的柑橘病毒性疾病，目前 CYVCV 在田间广泛分布。CYVCV 可通过嫁接传播，由柑橘木虱从柠檬传播到豆类，再从豆类传播到豆类。然而，到目前为止，还没有证据表明有任何媒介能将 CYVCV 从柑橘传播到柑橘。在这项研究中，在 24 小时的获得期（AAP）后，通过定量 RT-PCR 测试了柑橘木虱、柑橘全爪螨和柑橘粉虱对 CYVCV 的感染。在 48 小时的 AAP 后，将成螨组的柑橘木虱、柑橘全爪螨和柑橘粉虱给予 48 小时的接种期，在大柑橘酸橙苗上接种。在通过柑橘木虱传播后的 3、6 和 12 个月，在受体植物中检测到了 CYVCV，感染树的平均发病率分别为 31.9%、39.1%和 39.1%。柑橘全爪螨或柑橘粉虱均不能将 CYVCV 传播给柑橘。这是首次报道在实验室条件下，柑橘木虱能够将 CYVCV 从感染的柑橘传播到健康的柑橘。
