Paesani C, Salvucci E, Moiraghi M, Fernandez Canigia L, Pérez G T
Instituto de Ciencia y Tecnología en Alimentos Córdoba, (ICYTAC-CONICET-UNC), Córdoba, Argentina.
Laboratorio de Microbiología y Biotecnología, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, (FCEFYN-UNC), Cordoba, Argentina.
Lett Appl Microbiol. 2019 Feb;68(2):142-148. doi: 10.1111/lam.13097. Epub 2019 Jan 4.
Arabinoxylans are part of dietary fibre and have received attention given their emergent prebiotic character. Four arabinoxylans extracts were obtained from Argentinian soft and hard wheat. In vitro assays were performed to describe the extent to which the extracts from whole wheat flour support selective growth of Bifidobacterium breve and probiotic Lactobacillus reuteri ATCC23272 in a defined media. The prebiotic effect was evaluated by three quantitative scores: relative growth, prebiotic activity score and prebiotic index. For prebiotic index equation the growth of Bacteroides and Clostridium strains was compared to that of bifidobacteria and lactic acid bacteria. All the arabinoxylans extracts supported the growth of Lactobacillus and Bifidobacterium, reaching higher prebiotic activity score values than inulin (0·37 and 0·36 for Lactobacillus and Bifidobacterium respectively). AX2 from soft wheat and AX4 from hard showed similar prebiotic index value to commercial inulin (2·64, 2·52 and 2·22 respectively), and AX3 extract presented higher prebiotic index value (4·09) than the positive control and other prebiotic index reported for arabinoxylans. These extracts could be used as prebiotic, synbiotic compositions or novel food prototypes to treat dysbiosis associated with many diseases. SIGNIFICANCE AND IMPACT OF THE STUDY: The present work demonstrates that AX extracts from Argentinian soft and hard wheat promote efficiently the growth of probiotic strain L. reuteri ATCC23272 and B. breve 286, validated with three different parameters that consider the growth of representative strains of Bacteria genera found in the gut. The evaluation of AX extracts as a food supplement in a murine model could confirm their ability to modulate the microbiome. Novel food prototypes including AX and probiotics could relieve local symptoms and may act as psychobiotics with a beneficial effect on microbiome-brain axis.
阿拉伯木聚糖是膳食纤维的一部分，鉴于其新出现的益生元特性而受到关注。从阿根廷的软质小麦和硬质小麦中获得了四种阿拉伯木聚糖提取物。进行了体外试验，以描述全麦粉提取物在特定培养基中支持短双歧杆菌和益生菌罗伊氏乳杆菌ATCC23272选择性生长的程度。通过三个定量指标评估益生元效应：相对生长率、益生元活性评分和益生元指数。对于益生元指数方程，将拟杆菌属和梭菌属菌株的生长与双歧杆菌和乳酸菌的生长进行比较。所有阿拉伯木聚糖提取物均支持乳酸杆菌和双歧杆菌的生长，其益生元活性评分值高于菊粉（乳酸杆菌和双歧杆菌分别为0·37和0·36）。软质小麦的AX2和硬质小麦的AX4显示出与商业菊粉相似的益生元指数值（分别为2·64、2·52和2·22），AX3提取物的益生元指数值（4·09）高于阳性对照以及报道的其他阿拉伯木聚糖的益生元指数。这些提取物可用作益生元、合生元组合物或新型食品原型，以治疗与多种疾病相关的肠道菌群失调。研究的意义和影响：目前的工作表明，从阿根廷软质小麦和硬质小麦中提取的阿拉伯木聚糖提取物能有效促进益生菌罗伊氏乳杆菌ATCC23272和短双歧杆菌286的生长，通过三个不同参数进行验证，这些参数考虑了肠道中细菌属代表性菌株的生长情况。在小鼠模型中评估阿拉伯木聚糖提取物作为食品补充剂的效果，可以证实它们调节微生物群的能力。包括阿拉伯木聚糖和益生菌的新型食品原型可以缓解局部症状，并且可能作为精神益生菌对微生物群 - 脑轴产生有益影响。
