Pediatric Biology Center, Translational Health Science & Technology Institute, NCR Biotech Science Cluster, 3rd Milestone, Faridabad-Gurgaon Expressway, Faridabad, Haryana, India.
Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, United States of America.
PLoS One. 2018 Nov 16;13(11):e0207297. doi: 10.1371/journal.pone.0207297. eCollection 2018.
To compare immune phenotypes across two geographic and ethnic communities, we examined umbilical cord blood by flow cytometry and Luminex in parallel cohorts of 53 newborns from New Delhi, India, and 46 newborns from Stanford, California. We found that frequencies of a B cell subset suggested to be B-1-like, and serum IgM concentration were both significantly higher in the Stanford cohort, independent of differences in maternal age. While serum IgA levels were also significantly higher in the Stanford cohort, IgG1, IgG2, and IgG4 were significantly higher in the New Delhi samples. We found that neutrophils, plasmacytoid dendritic cells, CD8+ T cells, and total T cells were higher in the U.S. cohort, while dendritic cells, patrolling monocytes (CD14dimCD16+), natural killer cells, CD4+ T cells, and naïve B cells were higher in the India cohort. Within the India cohort, we also identified cell types whose frequency was positively or negatively predictive of occurrence of infection(s) in the first six months of life. Monocytes, total T cells, and memory CD4+ T cells were most prominent in having an inverse relationship with infection. We suggest that these data provide impetus for follow-up studies linking phenotypic differences to environmental versus genetic factors, and to infection outcomes.
为了比较两个地理和种族社区的免疫表型，我们通过流式细胞术和 Luminex 平行检测了来自印度新德里的 53 名新生儿和来自加利福尼亚斯坦福的 46 名新生儿的脐带血。我们发现，斯坦福队列中的 B 细胞亚群频率（提示为 B-1 样）和血清 IgM 浓度均显著更高，这与母体年龄差异无关。虽然斯坦福队列中的血清 IgA 水平也显著更高，但新德里样本中的 IgG1、IgG2 和 IgG4 水平显著更高。我们发现美国队列中的中性粒细胞、浆细胞样树突状细胞、CD8+T 细胞和总 T 细胞更高，而印度队列中的树突状细胞、巡逻单核细胞（CD14dimCD16+）、自然杀伤细胞、CD4+T 细胞和幼稚 B 细胞更高。在印度队列中，我们还确定了一些细胞类型，其频率与生命前 6 个月发生感染的情况呈正相关或负相关。单核细胞、总 T 细胞和记忆 CD4+T 细胞与感染的关系最为密切。我们认为这些数据为后续研究提供了动力，这些研究将表型差异与环境与遗传因素以及感染结果联系起来。
