Jilin Engineering Research Center for Agricultural Resources and Comprehensive Utilization, Jilin Institute of Chemical Technology, Jilin 132022, PR China; School of Pharmaceutical Sciences, Jilin University, Changchun 130012, PR China.
Jilin Engineering Research Center for Agricultural Resources and Comprehensive Utilization, Jilin Institute of Chemical Technology, Jilin 132022, PR China.
Int J Biol Macromol. 2019 Feb 15;123:280-290. doi: 10.1016/j.ijbiomac.2018.11.074. Epub 2018 Nov 13.
Seabuckthorn berries are rich in various bioactive components and used as a traditional medicine for a long time. Until now, little information is available for the extraction of polysaccharides from seabuckthorn berries (PSB) by linking antioxidant activity and microwave power. In this study, microwave-assisted extraction, characterization, in vitro and in vivo antioxidant activities of PSB were explored. The maximum PSB extraction yield of 0.264±0.005% was obtained under the optimal conditions as follows: microwave power 600W, extraction time 6min, liquid to material ratio 10: 1mL/g, and extraction temperature 85°C. Meanwhile, effects of microwave power on antioxidant activity of PSB was investigated and found that microwave at power of 600W can facilitate the release of antioxidant PSB in a high yield. The main monosaccharides of PSB were Rha, Man, Glu, and Gal at a molar ratio of 1.00: 6.89: 1.62: 13.52, UV and FT-IR analysis coupled with molecular weight determination further indicated that PSB is a polydisperse polysaccharide. Moreover, PSB obtained under the optimal conditions equally exerted in vivo antioxidant activity through decreasing malonaldehyde and protein carbonyls and increasing superoxide dismutase and glutathione.
沙棘果富含多种生物活性成分，长期以来一直被用作传统药物。到目前为止，关于通过抗氧化活性和微波功率连接从沙棘果中提取多糖（PSB）的信息很少。本研究探索了 PSB 的微波辅助提取、特性、体外和体内抗氧化活性。在最佳条件下，PSB 的最大提取产率为 0.264±0.005%，条件为：微波功率 600W、提取时间 6min、液料比 10:1mL/g、提取温度 85°C。同时，考察了微波功率对 PSB 抗氧化活性的影响，发现 600W 的微波功率可以促进高产量的抗氧化 PSB 的释放。PSB 的主要单糖为鼠李糖、甘露糖、葡萄糖和半乳糖，摩尔比为 1.00:6.89:1.62:13.52，紫外和傅里叶变换红外分析结合分子量测定进一步表明 PSB 是一种多分散多糖。此外，在最佳条件下获得的 PSB 通过降低丙二醛和蛋白质羰基，增加超氧化物歧化酶和谷胱甘肽，同样发挥了体内抗氧化活性。
