School of Bioscience and Biopharmaceutics, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, Guangdong Pharmaceutical University, 510006 Guangzhou, Guangdong, China.
School of Bioscience and Biopharmaceutics, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, Guangdong Pharmaceutical University, 510006 Guangzhou, Guangdong, China.
J Ethnopharmacol. 2019 Mar 1;231:90-97. doi: 10.1016/j.jep.2018.11.018. Epub 2018 Nov 14.
Danggui Buxue Tang has been used in China to treat clinical anemia for more than 800 years. However, there is no scientific report on its effect on bone marrow stromal cells.
Here, we aimed to explore the effect of Danggui Buxue Tang on bone marrow stromal cell adhesion and migration.
Bone marrow stromal cells were used as a model to evaluate the effect of Danggui Buxue Tang on the adhesion and migration of bone marrow stromal cells. RNA-sequencing, quantitative polymerase chain reaction, and western blotting were used to detect and confirm the expression of genes related to the focal adhesion pathway before and after drug delivery.
Danggui Buxue Tang significantly increased the number of bone marrow stromal cells. After 12 days of 16 mg/mL Danggui Buxue Tang treatment, bone marrow stromal cells were significantly increased (by 0.527 ± 0.008 fold; p < 0.001) as compared to the control group (0.180 ± 0.019). The effect was not due to enhanced cell proliferation, as there was no difference in the cell cycle (p > 0.05). The adhesion area of a single cell was doubled by Danggui Buxue Tang treatment (p < 0.001), and the time required for cell adhesion to a Petri dish was shortened. Thus, Danggui Buxue Tang increases the number of bone marrow stromal cells by promoting adhesion. Danggui Buxue Tang also significantly promoted bone marrow stromal cell migration (p < 0.001). Transcript analysis revealed that the focal adhesion and PI3K-Akt signaling pathways were activated. Expression analysis confirmed that the gene and protein expression of focal adhesion-related factors were upregulated.
Danggui Buxue Tangaffects bone marrow stromal cell adhesion and migration by enhancing the focal adhesion pathway in vitro, and bone marrow stromal cells are a target of DBT-regulated hematopoiesis, and the active ingredients of DBT involved in the effects require further investigation.
当归补血汤在中国已被用于治疗临床贫血 800 多年。然而，目前尚无关于其对骨髓基质细胞影响的科学报告。
本研究旨在探讨当归补血汤对骨髓基质细胞黏附和迁移的影响。
以骨髓基质细胞为模型，评价当归补血汤对骨髓基质细胞黏附和迁移的影响。采用 RNA 测序、定量聚合酶链反应和 Western blot 检测和确认药物给药前后与粘着斑通路相关的基因表达。
当归补血汤显著增加了骨髓基质细胞的数量。在 16mg/ml 当归补血汤处理 12 天后，与对照组（0.180±0.019）相比，骨髓基质细胞明显增加（增加 0.527±0.008 倍；p<0.001）。这种作用不是由于增强细胞增殖，因为细胞周期没有差异（p>0.05）。当归补血汤处理后单个细胞的黏附面积增加了一倍（p<0.001），并且细胞黏附到培养皿所需的时间缩短。因此，当归补血汤通过促进黏附来增加骨髓基质细胞的数量。当归补血汤还显著促进了骨髓基质细胞的迁移（p<0.001）。转录分析显示，粘着斑和 PI3K-Akt 信号通路被激活。表达分析证实，与粘着斑相关的因子的基因和蛋白表达上调。
当归补血汤通过体外增强粘着斑通路影响骨髓基质细胞的黏附和迁移，骨髓基质细胞是 DBT 调节造血的靶细胞，DBT 中涉及作用的活性成分需要进一步研究。
