Department of Analytical Chemistry, Faculty of Chemistry, University of Gdansk, 63 Wita Stwosza St., 80-308 Gdansk, Poland.
Department of Analytical Chemistry, Faculty of Chemistry, University of Gdansk, 63 Wita Stwosza St., 80-308 Gdansk, Poland.
Biosens Bioelectron. 2019 Feb 1;126:308-314. doi: 10.1016/j.bios.2018.10.063. Epub 2018 Oct 31.
Two different type of electrodes, boron-doped diamond electrode (BDD) and boron-doped carbon nanowalls (B:CNW) electrode, were used for the electrochemical determination of paracetamol using the cyclic voltammetry and the differential pulse voltammetry in phosphate buffered saline, pH = 7.0. The main advantage of these electrodes is their utilization without any additional modification of the electrode surface. The peak current was linearly related to the concentration of paracetamol in the range from 0.065 µM to 32 µM for BDD electrode and from 0.032 µM to 32 µM for B:CNW electrode. The limit of detection was 0.430 µM and 0.281 µM for BDD and B:CNW electrode, respectively. Additionally, we studied the effect of pH on the redox reaction of paracetamol at the both electrodes in Britton-Robinson buffer solution in the range of pH 3.0-12.0, indicating the pH 7.0 value as the most suitable for the current experiments. The studies also included the various scan rates in range of 50-500 mV/s. Finally, our team selected the B:CNW electrode for the determination of paracetamol in the artificial urine sample using differential pulse voltammetry method, obtaining the calculated limit of detection on the level of 0.08006 µM.
两种不同类型的电极，掺硼金刚石电极（BDD）和掺硼碳纳米墙电极（B:CNW），被用于在磷酸盐缓冲溶液（pH = 7.0）中使用循环伏安法和差分脉冲伏安法测定扑热息痛。这些电极的主要优点是可以在无需对电极表面进行任何额外修饰的情况下使用。BDD 电极的峰电流与扑热息痛浓度在 0.065 µM 至 32 µM 范围内呈线性关系，B:CNW 电极的峰电流与扑热息痛浓度在 0.032 µM 至 32 µM 范围内呈线性关系。BDD 和 B:CNW 电极的检测限分别为 0.430 µM 和 0.281 µM。此外，我们还在 Britton-Robinson 缓冲溶液中研究了 pH 值对两种电极上扑热息痛氧化还原反应的影响，结果表明 pH 值 7.0 最适合当前实验。研究还包括在 50-500 mV/s 范围内的不同扫描速率。最后，我们的团队选择了 B:CNW 电极用于使用差分脉冲伏安法测定人工尿液样品中的扑热息痛，获得的计算检测限为 0.08006 µM。
