Division of Infectious Diseases, Department of Internal Medicine, The University of Texas Health Science Center at Houston, 6431 Fannin St. Houston, TX 77030, USA.
UTHealth's Center for Antimicrobial Resistance and Microbial Genomics (CARMiG), 6431 Fannin St., Houston, TX 77030.
Pathog Dis. 2018 Nov 1;76(8):fty084. doi: 10.1093/femspd/fty084.
Ace (Adhesin to collagen from Enterococcus faecalis) is a cell-wall anchored protein that is expressed conditionally and is important for virulence in a rat infective endocarditis (IE) model. Previously, we showed that rats immunized with the collagen binding domain of Ace (domain A), or administered anti-Ace domain A polyclonal antibody, were less susceptible to E. faecalis endocarditis than sham-immunized controls. In this work, we demonstrated that a sub nanomolar monoclonal antibody (mAb), anti-Ace mAb70, significantly diminished E. faecalis binding to ECM collagen IV in in vitro adherence assays and that, in the endocarditis model, anti-Ace mAb70 pre-treatment significantly reduced E. faecalis infection of aortic valves. The effectiveness of anti-Ace mAb against IE in the rat model suggests it might serve as a beneficial agent for passive protection against E. faecalis infections.
Ace（来自粪肠球菌的胶原黏附素）是一种细胞壁锚定蛋白，条件表达，对大鼠感染性心内膜炎（IE）模型的毒力很重要。以前，我们发现用 Ace 的胶原结合结构域（域 A）免疫的大鼠或给予抗 Ace 域 A 多克隆抗体的大鼠比假免疫对照组更不易受粪肠球菌心内膜炎的影响。在这项工作中，我们证明了一种亚纳摩尔单克隆抗体（mAb），抗 Ace mAb70，在体外黏附试验中显著减少了粪肠球菌与 ECM 胶原 IV 的结合，并且在心内膜炎模型中，抗 Ace mAb70 预处理显著降低了粪肠球菌对主动脉瓣的感染。抗 Ace mAb 对大鼠模型中 IE 的有效性表明，它可能作为一种有益的被动保护剂，对抗粪肠球菌感染。
