Department of Chemistry and Biochemistry, Lamar University, Beaumont, TX 77710, USA.
Molecules. 2018 Nov 15;23(11):2979. doi: 10.3390/molecules23112979.
Zinc Oxide (ZnO) nanoparticles are suspected to produce toxic effects toward mammalian cells; however, discrepancies in the extent of this effect have been reported between different cell lines. Simultaneously, high levels of ultraviolet (UV-C) radiation can have carcinogenic effects. The mechanism of this effect is also not well understood. Due to similarities in phenotype morphology after cell exposure to ZnO nanoparticles and UV-C irradiation, we emit the hypothesis that the toxicity of both these factors is related to damage of cellular membranes and affect their sterol content. Wild-type Chinese Hamster Ovary (CHO-K1) cells were exposed to ZnO nanoparticles or UV-C radiation. The amount of absorbed ZnO was determined by UV-visible spectroscopy and the changes in sterol profiles were evaluated by gas chromatography. Cell viability after both treatments was determined by microscopy. Comparing morphology results suggested similarities in toxicology events induced by ZnO nanoparticles and UV exposure. UV-C exposure for 360 min disrupts the sterol metabolic pathway by increasing the concentration of cholesterol by 21.6-fold. This increase in cholesterol production supports the hypothesis that UV irradiation has direct consequences in initiating sterol modifications in the cell membrane.
氧化锌 (ZnO) 纳米粒子被怀疑对哺乳动物细胞产生毒性作用；然而，不同细胞系之间的这种作用的程度存在差异。同时，高水平的紫外线 (UV-C) 辐射会产生致癌作用。这种效应的机制也不是很清楚。由于细胞暴露于 ZnO 纳米粒子和 UV-C 辐射后的表型形态相似，我们提出假设，这两种因素的毒性都与细胞膜的损伤有关，并影响其固醇含量。野生型中国仓鼠卵巢 (CHO-K1) 细胞暴露于 ZnO 纳米粒子或 UV-C 辐射下。通过紫外可见光谱测定吸收的 ZnO 量，并通过气相色谱法评估固醇谱的变化。通过显微镜观察确定两种处理后的细胞活力。比较形态学结果表明，ZnO 纳米粒子和 UV 暴露引起的毒理学事件存在相似性。UV-C 暴露 360 分钟会通过将胆固醇浓度增加 21.6 倍来破坏固醇代谢途径。胆固醇产量的增加支持了这样的假设，即 UV 辐射会直接导致细胞膜中固醇的修饰。
