酿酒酵母钾激活醛脱氢酶的动力学及反应机制
Kinetics and reaction mechanism of potassium-activated aldehyde dehydrogenase from Saccharomyces cerevisiae.
作者信息
Bostian K A, Betts G F
出版信息
Biochem J. 1978 Sep 1;173(3):787-98. doi: 10.1042/bj1730787.
Abstract
Data from steady-state kinetic analysis of yeast K+-activated aldehyde dehydrogenase are consistent with a ternary complex mechanism. Evidence from alternative substrate analysis and product-inhibition studies supports an ordered sequence of substrate binding in which NAD+ is the leading substrate. A preincubation requirement for NAD+ for maximum activity is also consistent with the importance of a binary enzyme-NAD+ complex. Dissociation constant for enzyme-NAD+ complex determined kinetically is in reasonable agreement with that determined by direct binding. The order of substrate addition proposed here differs from that proposed for a yeast aldehyde dehydrogenase previously reported. Different methods of purification produced an enzyme that showed similar kinetic characteristics to those reported here.
摘要
酵母K⁺激活的醛脱氢酶稳态动力学分析的数据与三元复合物机制一致。来自替代底物分析和产物抑制研究的证据支持底物结合的有序序列，其中NAD⁺是主要底物。NAD⁺预孵育以达到最大活性的要求也与二元酶-NAD⁺复合物的重要性一致。通过动力学测定的酶-NAD⁺复合物的解离常数与通过直接结合测定的解离常数合理吻合。这里提出的底物添加顺序与先前报道的酵母醛脱氢酶的顺序不同。不同的纯化方法产生的一种酶，其动力学特征与这里报道的相似。
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本文引用的文献
1
ON THE SITES OF ATTACHMENT AND REACTION OF ALDEHYDE DEHYDROGEN ASES.论醛脱氢酶的附着位点及反应
Proc Natl Acad Sci U S A. 1960 Feb;46(2):206-12. doi: 10.1073/pnas.46.2.206.
2
Yeast aldehyde dehydrogenase.酵母乙醛脱氢酶
Arch Biochem Biophys. 1951 Nov;34(1):86-97. doi: 10.1016/s0003-9861(51)80013-4.
3
The kinetics of enzyme-catalyzed reactions with two or more substrates or products. III. Prediction of initial velocity and inhibition patterns by inspection.双底物或多底物及多产物酶催化反应的动力学。III. 通过观察预测初速度和抑制模式
Biochim Biophys Acta. 1963 Feb 12;67:188-96. doi: 10.1016/0006-3002(63)91816-x.
4
The kinetics of enzyme-catalyzed reactions with two or more substrates or products. II. Inhibition: nomenclature and theory.具有两种或更多种底物或产物的酶催化反应动力学。II. 抑制作用：命名法与理论
Biochim Biophys Acta. 1963 Feb 12;67:173-87. doi: 10.1016/0006-3002(63)91815-8.
5
The kinetics of enzyme-catalyzed reactions with two or more substrates or products. I. Nomenclature and rate equations.具有两种或更多种底物或产物的酶催化反应动力学。I. 命名法和速率方程。
Biochim Biophys Acta. 1963 Jan 8;67:104-37. doi: 10.1016/0006-3002(63)91800-6.
6
Kinetic formulations for enzymic reactions involving two substrates.涉及两种底物的酶促反应的动力学公式。
Can J Biochem Physiol. 1962 Jun;40:763-804.
7
Aldehyde oxidation. V. Direct conversion of malonic semialdehyde to acetyl-coenzyme A.醛氧化。V. 丙二酸半醛直接转化为乙酰辅酶A。
J Biol Chem. 1960 Mar;235:589-94.
8
Aldehyde oxidation. III. Succinic semialdehyde dehydrogenase.醛氧化。III. 琥珀酸半醛脱氢酶。
J Biol Chem. 1959 Apr;234(4):937-40.
9
Aldehyde oxidation. I. Dehydrogenase from Pseudomonas fluorescens.醛氧化。I. 荧光假单胞菌中的脱氢酶。
J Biol Chem. 1958 May;232(1):75-87.
10
Effects of univalent cations on the properties of yeast NAD+ acetaldehyde dehydrogenase.单价阳离子对酵母NAD⁺乙醛脱氢酶性质的影响。
Biochim Biophys Acta. 1966 Apr 12;118(1):1-8. doi: 10.1016/s0926-6593(66)80139-x.
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