Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; Beijing Advanced Innovation Centre for Biomedical Engineering, Beihang University, Beijing 102402, China; Shenzhen Research Institute, Beihang University, Shenzhen 518057, China.
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; Beijing Advanced Innovation Centre for Biomedical Engineering, Beihang University, Beijing 102402, China; Shenzhen Research Institute, Beihang University, Shenzhen 518057, China.
Int J Biol Macromol. 2019 Feb 15;123:622-628. doi: 10.1016/j.ijbiomac.2018.11.099. Epub 2018 Nov 14.
The property of biomaterials to control the sequential release of growth factors has been widely concerned in the field of bone repair and regeneration. Double-factor sustained-release scaffolds have good biocompatibility and bioactivity. O‑Carboxymethyl chitosan microspheres (O-CMCS) has antimicrobial activity, biodegradability, biocompatibility and film formation. This study developed scaffolds materials with microspheres with a purpose of forming a controlled slow release secondary structure. O-CMCS were used as drug carrier to construct the compound sustained-release system with rhBMP-2 and VEGF double factors release. The O-CMCS were loaded with rhBMP-2 and the hydroxyapatite collagen (HC) scaffolds were loaded with VEGF to prepare the scaffolds. These were double factors composite sustained-release system scaffolds. The biocompatibility of the sustained-release system was evaluated by in vitro and in vivo experiments. HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments. The results showed that rhBMP-2/VEGF in HC composite scaffolds (HCBV) successfully achieved the sequential release of the double factors, which could benefit bone regeneration.
生物材料控制生长因子顺序释放的特性在骨修复和再生领域受到广泛关注。双因子缓释支架具有良好的生物相容性和生物活性。O-羧甲基壳聚糖微球（O-CMCS）具有抗菌活性、可生物降解性、生物相容性和成膜性。本研究开发了具有微球的支架材料，目的是形成控制缓慢释放的二级结构。将 O-CMCS 用作药物载体，构建 rhBMP-2 和 VEGF 双因素释放的复合缓释系统。将 O-CMCS 负载 rhBMP-2，将羟基磷灰石胶原（HC）支架负载 VEGF，制备支架。这些是双因素复合缓释系统支架。通过体外和体内实验评价缓释系统的生物相容性。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、X 射线衍射检测（XRD）、体外释放试验、体外细胞培养和体内动物实验对 HC 复合支架进行了表征。结果表明，HC 复合支架（HCBV）中的 rhBMP-2/VEGF 成功实现了双因素的顺序释放，这有利于骨再生。
