带电表面活性剂能否展开不带电的蛋白质？
Can a Charged Surfactant Unfold an Uncharged Protein?
机构信息
Linderstrøm-Lang Centre for Protein Science, Department of Biology, University of Copenhagen, Copenhagen, Denmark.
Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, Denmark.
出版信息
Biophys J. 2018 Dec 4;115(11):2081-2086. doi: 10.1016/j.bpj.2018.10.022. Epub 2018 Nov 15.
Abstract
Does sodium dodecyl sulfate (SDS) denature proteins through electrostatic SDS-protein interactions? We show that a protein completely lacking charged side chains is unfolded by SDS in a manner similar to charged proteins, revealing that formal protein charges are not required for SDS-induced protein unfolding or binding.
摘要
十二烷基硫酸钠（SDS）是否通过静电 SDS-蛋白质相互作用使蛋白质变性？我们表明，一种完全缺乏带电侧链的蛋白质在 SDS 的作用下展开，其方式类似于带电蛋白质，这表明 SDS 诱导蛋白质展开或结合不需要正式的蛋白质电荷。
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