Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki, 305-0856, Japan.
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
Arch Microbiol. 2019 Jul;201(5):571-579. doi: 10.1007/s00203-018-1596-x. Epub 2018 Nov 17.
Campylobacter jejuni is one of the most important causes of food-borne diseases in industrialized countries. Amino acids are an important nutrient source for this pathogen because it lacks enzymes related to glycolysis. However, the metabolic characteristics of C. jejuni grown in a nutrient-restricted medium with specific amino acids have not been fully elucidated. This study shows that C. jejuni NCTC 11168 grows well in a nutrient-restricted medium containing serine, aspartate, glutamate, and proline. Subtracting serine significantly reduced growth, but the removal of the three other amino acids did not, suggesting that serine is a priority among the four amino acids. A transcriptomic analysis of C. jejuni NCTC 11168 grown in a medium with serine as the main energy source was then performed. Serine seemed to be sensed by some chemoreceptors, and C. jejuni reached an adaptation stage with active growth in which the expression of flagellar assembly components was downregulated and the biosyntheses of multiple amino acids and nucleotide sugars were upregulated. These data suggest that C. jejuni NCTC 11168 requires serine as a nutrient.
空肠弯曲菌是工业化国家最重要的食源性病原体之一。由于该病原体缺乏与糖酵解相关的酶，因此氨基酸是其重要的营养来源。然而，在含有特定氨基酸的营养受限培养基中生长的空肠弯曲菌的代谢特征尚未完全阐明。本研究表明，空肠弯曲菌 NCTC 11168 在含有丝氨酸、天冬氨酸、谷氨酸和脯氨酸的营养受限培养基中生长良好。减去丝氨酸会显著降低生长速度，但去除其他三种氨基酸则不会，这表明丝氨酸是这四种氨基酸中的优先选择。然后对以丝氨酸为主要能源的空肠弯曲菌 NCTC 11168 进行了转录组分析。丝氨酸似乎被某些化学感受器感知，空肠弯曲菌进入适应阶段，表现为活跃生长，鞭毛组装成分的表达下调，多种氨基酸和核苷酸糖的生物合成上调。这些数据表明空肠弯曲菌 NCTC 11168 需要丝氨酸作为营养物质。
