Letcher Peter M, Powell Martha J
Department of Biological Sciences, The University of Alabama, Tuscaloosa, AL, 35487, USA.
Fungal Biol. 2018 Dec;122(12):1171-1183. doi: 10.1016/j.funbio.2018.08.003. Epub 2018 Aug 24.
The purpose of our research is to investigate the morphology, zoospore ultrastructure, and molecular phylogenetic placement of a chytrid from Australia. From a survey of chytrid fungi in New South Wales, Australia, we isolated strain PL AUS 026 and putatively identified it as Polyphlyctis unispina. Light microscopic evaluation determined strain PL AUS 026 to be similar to two other strains of P. unispina characterized in the literature but to have a more complex thallus than that of the type. Molecular phylogenetic analyses placed our strain as sister of or basal to Chytridiaceae, Chytridiales. Ultrastructural analysis of the zoospore of strain PL AUS 026 revealed unique features. On the basis of our analyses we designate strain PL AUS 026 as a new species, Polyphlyctis willoughbyi. This research extends our concept of Chytridiaceae systematics and ultrastructural variation in the Chytridiales zoospore.
我们研究的目的是调查一种来自澳大利亚的壶菌的形态、游动孢子超微结构以及分子系统发育位置。通过对澳大利亚新南威尔士州的壶菌进行调查，我们分离出了菌株PL AUS 026，并初步将其鉴定为单棘多壶菌。光学显微镜评估确定菌株PL AUS 026与文献中描述的另外两种单棘多壶菌菌株相似，但菌体比模式菌株更复杂。分子系统发育分析将我们的菌株置于壶菌目壶菌科的姐妹分支或基部位置。对菌株PL AUS 026游动孢子的超微结构分析揭示了独特的特征。基于我们的分析，我们将菌株PL AUS 026指定为一个新物种，即威洛比多壶菌。这项研究扩展了我们对壶菌科系统学以及壶菌目游动孢子超微结构变异的认识。
