牛肝中3-羟基-3-甲基戊二酰辅酶A合酶的一些特性
Some properties of 3-hydroxy-3-methylglutaryl-coenzyme A synthase from ox liver.
作者信息
Page M A, Tubbs P K
出版信息
Biochem J. 1978 Sep 1;173(3):925-8. doi: 10.1042/bj1730925.
Abstract
Mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase (EC 4.1.3.5) was purified from ox liver, and obtained essentially free from 3-oxoacyl-CoA thiolases. The kinetic behaviour, like that of the synthases from chicken liver and yeast, is compatible with a reaction pathway involving condensation of an acetyl-enzyme with acetoacetyl-CoA. The Km for acetoacetyl-CoA, less than 1 micronM at pH 7.8, may possibly be low enough to permit rapid ketogenesis under physiological conditions without the need for a binary complex between the synthase and oxoacyl-CoA thiolase.
摘要
线粒体3-羟基-3-甲基戊二酰辅酶A合酶（EC 4.1.3.5）从牛肝中纯化得到，基本上不含3-氧代酰基辅酶A硫解酶。其动力学行为与鸡肝和酵母中的合酶相似，符合涉及乙酰化酶与乙酰乙酰辅酶A缩合的反应途径。在pH 7.8时，乙酰乙酰辅酶A的Km小于1微摩尔，可能低到足以在生理条件下实现快速生酮作用，而无需合酶与氧代酰基辅酶A硫解酶之间形成二元复合物。
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