Erekat Nour S
Department of Anatomy, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.
J Cell Death. 2018 Nov 5;11:1179066018809091. doi: 10.1177/1179066018809091. eCollection 2018.
Active caspase-3-mediated apoptosis has been implicated in the pathogenesis of harmaline-induced tremor. The aim of this study is to illustrate the impact of tremor induction on the expression of factors mediating the cell surface death receptor-dependent apoptosis. A total of 20 normal Wistar rats were randomly selected and equally divided into control and experimental groups. Tremor was induced in the experimental group by injecting the rats with a single dose of harmaline (50 mg/kg). After that, cerebellar tissues were evaluated by immunohistochemistry to examine the expression of tumor necrosis factor α (TNF-α) and active caspase-8 in the 2 groups of animals. TNF-α and active caspase-8 expression was significantly higher in cerebella from experimental rats compared with that in those from the control rats ( value < .01). Thus, our present data suggest the association of tremor induction with the cerebellar overexpression of TNF-α and active caspase-8, correlative with Purkinje cell (PC) loss indicated by loss of calbindin immunoreactivity, indicating the induction of the cell surface death receptor-mediated apoptosis.
活性半胱天冬酶-3介导的细胞凋亡与骆驼蓬碱诱发的震颤的发病机制有关。本研究的目的是阐明震颤诱导对介导细胞表面死亡受体依赖性细胞凋亡的因子表达的影响。总共随机选择20只正常Wistar大鼠，并将其平均分为对照组和实验组。通过给大鼠注射单剂量的骆驼蓬碱（50mg/kg）在实验组中诱发震颤。之后，通过免疫组织化学评估小脑组织，以检查两组动物中肿瘤坏死因子α（TNF-α）和活性半胱天冬酶-8的表达。与对照组大鼠相比，实验组大鼠小脑中TNF-α和活性半胱天冬酶-8的表达明显更高（P值<0.01）。因此，我们目前的数据表明震颤诱导与小脑TNF-α和活性半胱天冬酶-8的过表达相关，这与钙结合蛋白免疫反应性丧失所表明的浦肯野细胞（PC）丢失相关，表明细胞表面死亡受体介导的细胞凋亡被诱导。
