ABTS 过氧化物酶活性测试中的陷阱：光化过程的干扰。
Pitfalls in the ABTS Peroxidase Activity Test: Interference of Photochemical Processes.
机构信息
Departamento de Ciencia de los Materiales e Ingeniería Metalúrgica y Química Inorgánica, Facultad de Ciencias , Universidad de Cádiz , Avda. República Saharahui s/n , Puerto Real , 11510 Cádiz , Spain.
Departamento de Química Inorgánica, Facultade de Ciencias, Campus de Lugo , Universidade de Santiago de Compostela , Avda. Alfonso X s/n , Lugo 27002 , Spain.
出版信息
Inorg Chem. 2018 Dec 3;57(23):14471-14475. doi: 10.1021/acs.inorgchem.8b02525. Epub 2018 Nov 19.
ABTS (2,2'-azinobis(3-ethylbenzothiazoline)-6-sulfonic acid) oxidation to form its radical cation in the presence of HO is frequently used as a test for determining the peroxidase activity of enzyme mimics. Detailed studies using salen-type Mn(III) complexes show that photochemical processes involving HO, ABTS, and the complex itself can lead to erroneous results. The capability of the complexes to act as OH scavengers can be also relevant when the mechanism of their biological activity is considered.
ABTS（2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸））在 HO 的存在下氧化形成其自由基阳离子，常用于测定酶模拟物的过氧化物酶活性。使用西佛碱型 Mn(III)配合物的详细研究表明，涉及 HO、ABTS 和配合物本身的光化学过程可能导致错误的结果。当考虑其生物活性的机制时，配合物作为 OH 清除剂的能力也可能相关。
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