一种C-二鸟苷酸-硫酸普罗黄素-血红素超分子复合物具有过氧化物酶活性——对简单比色检测的启示。
A C-di-GMP-proflavine-hemin supramolecular complex has peroxidase activity--implication for a simple colorimetric detection.
作者信息
Nakayama Shizuka, Roelofs Kevin, Lee Vincent T, Sintim Herman O
机构信息
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
出版信息
Mol Biosyst. 2012 Mar;8(3):726-9. doi: 10.1039/c2mb05430b. Epub 2012 Jan 18.
Herein, we demonstrate that the bacterial signaling molecule, c-di-GMP, can enhance the peroxidation of hemin when proflavine is present. The c-di-GMP-proflavine-hemin nucleotidezyme can oxidize the colorless compound 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), ABTS, to the colored radical cation ABTS˙(+) and hence provides simple colorimetric detection of c-di-GMP at low micromolar concentrations.
在此，我们证明，当存在原黄素时，细菌信号分子环二鸟苷酸（c-di-GMP）可增强血红素的过氧化作用。c-di-GMP-原黄素-血红素核酶可将无色化合物2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）氧化为有色自由基阳离子ABTS˙(+)，因此可在低微摩尔浓度下对c-di-GMP进行简单的比色检测。
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