Department of Andrology, Dongzhimen Hospital Attached to Beijing University of Chinese Medicine , Dongcheng District , Beijing , China.
Department of Chinese Medicine, Beijing University of Chinese Medicine , Chaoyang District , Beijing , China.
Aging Male. 2019 Dec;22(4):278-286. doi: 10.1080/13685538.2018.1484443. Epub 2018 Nov 19.
The study was aimed to evaluate the influences of erectile dysfunction (ED) in a rat model of stroke combined with hyperlipidemia (HLP). Male Sprague-Dawley rats were divided into control and hyperlipidemia (HLP) groups. HLP model was constructed by feeding with high-fat and cholesterol diets. Serum levels of total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL), triglyceride (TG), and non-HDL were identified to check the model was success. Stroke model was established by FeCl. ICP/MAP value was detected to evaluate the erectile function of rats. Serum level of lipoproteins and the expressions of endothelial nitric oxide synthase (eNOS), vascular endothelial growth factor (VEGF) were detected by ELISA. Hematoxylin-eosin (HE) staining of corpus cavernosum and measurement of penis length were utilized to assessment erectile function. Western blot was used. TC, TG, LDL, and non-HDL-C in serum were up-regulated, while HDL level was attenuated. After treatment, the serum lipid level recovered. From the ICP/MAP values, the erectile function of both two treatment groups recovered. The expression of PDE5A was up-regulated, while the levels of eNOS and cGMP were suppressed after surgery. The length of penis was decreased, and corpus cavernosum was damaged following HLP and stroke. However, the erectile function was recovered after treatment. Stroke combined HLP caused ED through NO-cGMP-PDE5 pathway.
本研究旨在评估勃起功能障碍（ED）对合并高脂血症（HLP）的卒中大鼠模型的影响。雄性 Sprague-Dawley 大鼠分为对照组和高脂血症（HLP）组。HLP 模型通过高脂和胆固醇饮食喂养构建。检测总胆固醇（TC）、低密度脂蛋白（LDL）、高密度脂蛋白（HDL）、甘油三酯（TG）和非 HDL 的血清水平，以检查模型是否成功。通过 FeCl 建立卒中模型。通过检测颅内压（ICP）/平均动脉压（MAP）值来评估大鼠的勃起功能。通过 ELISA 检测脂蛋白血清水平和内皮型一氧化氮合酶（eNOS）、血管内皮生长因子（VEGF）的表达。通过苏木精-伊红（HE）染色检测海绵体和阴茎长度测量评估勃起功能。采用 Western blot 法。血清 TC、TG、LDL 和非 HDL-C 水平上调，而 HDL 水平降低。治疗后，血清脂质水平恢复。从 ICP/MAP 值来看，两种治疗组的勃起功能均恢复。手术后 PDE5A 的表达上调，而 eNOS 和 cGMP 的水平受到抑制。阴茎长度缩短，海绵体受损，合并 HLP 和卒中后。然而，治疗后勃起功能得到恢复。卒中合并 HLP 通过 NO-cGMP-PDE5 通路引起 ED。
