Immunology Department, "Victor Babes" National Institute of Pathology, Bucharest, Romania.
Pathology Department, "Colentina" Clinical Hospital, Bucharest, Romania.
J Cell Mol Med. 2019 Feb;23(2):1086-1094. doi: 10.1111/jcmm.14009. Epub 2018 Nov 19.
Phthalocyanines (Pc) and their metallated derivatives are strongly considered for photodynamic therapy (PDT) possessing unique properties as possible new photosensitizers (PS). We have used toxicological assessments, real-time monitoring of cellular impedance, and imagistic measurements for assessing the in vitro dark toxicity and PDT efficacy of Ga(III)-Pc in SHSy5Y neuroblastoma cells. We have established the non-toxic concentration range of Ga(III)-Pc, a compound which shows a high intracellular accumulation, with perinuclear distribution in confocal microscopy. By choosing Ga(III)Pc non-toxic dose, we performed in vitro experimental PDT hampering cellular proliferation. Our proposed Ga(III)-Pc could complete a future PS panel for neuroblastoma alternate therapy.
酞菁（Pc）及其金属衍生物被强烈认为是光动力疗法（PDT）的候选药物，具有作为新型光敏剂（PS）的独特特性。我们使用毒理学评估、细胞阻抗的实时监测和成像测量来评估 Ga(III)-Pc 在 SHSy5Y 神经母细胞瘤细胞中的体外暗毒性和 PDT 疗效。我们确定了 Ga(III)-Pc 的无毒浓度范围，该化合物在共聚焦显微镜下显示出高细胞内积累和核周分布。通过选择 Ga(III)Pc 的无毒剂量，我们进行了体外实验性 PDT 以抑制细胞增殖。我们提出的 Ga(III)-Pc 可以为神经母细胞瘤的替代疗法完成未来的 PS 面板。
