Wallace G R, McLeod A, Chain B M
Department of Biology, University College, London, U.K.
J Chromatogr. 1988 Jun 3;427(2):239-46. doi: 10.1016/0378-4347(88)80126-9.
The insulin molecule was derivatised by reaction with trinitrobenzenesulphonic acid (TNBS), which is known to react predominantly with free primary amino groups. The products of the reaction were analysed by reversed-phase chromatography and by further derivatisation with dansyl chloride. Under the conditions of these experiments, TNBS was found to react preferentially with glycine at position A1. This finding is discussed in terms of the tertiary structure and immunogenicity of this derivative.
胰岛素分子通过与三硝基苯磺酸（TNBS）反应进行衍生化，已知该物质主要与游离的伯氨基反应。反应产物通过反相色谱法以及用丹磺酰氯进一步衍生化进行分析。在这些实验条件下，发现TNBS优先与A1位的甘氨酸反应。根据该衍生物的三级结构和免疫原性对这一发现进行了讨论。
